Lunaphore Technologies Sa

Ecole Polytechnique Federale De Lausanne

Ecole Polytechnique Féderale De Lausanne (epfl)

Diego Gabriel Dupouy

Ata Tuna Ciftlik

Pierre Joris

Marco Ammann

Martin Gijs

Marta Comino

Giulia Cappi

Deniz Eroglu

Deborah Heintze

Déborah Heintze

Benjamin Pelz

Biological sample processing system comprising an imaging unit () comprising a digital image processing system and at least one microscope including at least one lens (), a sample processing station () comprising a handling platform () including a support () and a displacement mechanism for moving the support (), and a sample processing unit () mounted on the handling platform (). The sample processing unit () comprises a tissue slide holder () for mounting thereon a tissue slide () with a biological sample () fixed thereon and a microfluidic cartridge holder () for mounting a microfluidic cartridge () thereon. The tissue slide holder () is coupled to the microfluidic cartridge holder () via a coupling () allowing the microfluidic cartridge and the tissue support to be mounted and removed from the sample processing unit in an opened position, and in a closed position for the tissue support () to be in sealing contact with the microfluidic cartridge (). The sample processing station comprises a plurality of said sample processing units mounted on the handling platform () and moveable from a position allowing mounting of the tissue slide, respectively microfluidic cartridge, or removal thereof, to a position in which the viewing window in the microfluidic cartridge holder () is positioned in alignment with the lens of said at least one microscope.

Biological sample processing system and microfluidic cartridge therefor

The invention relates to a device and method for in situ temperature-induced antigen retrieval of samples wherein all steps are performed under a pressure higher than the atmospheric pressure on a sample immobilized on a sample support which can be further subjected to staining and imaging on the same sample support, optionally by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner.

Methods of in situ antigen retrieval of a biological sample and imaging thereof

Microfluidic network device () configured to supply reagents to a biological tissue sampling device (), comprising a plurality of microfluidic inlet channels () connected to respective sources of said reagents, at least one common outlet channel (), and a plurality of valves () interconnecting an outlet end () of each of said plurality of inlet channels to said at least one common outlet channel.

Microfluidic network device

Microfluidic cartridge () comprising a sampling device () having a sealing ring () arranged to form a microfluidic chamber () when a support containing a biological sample is brought into contact with the sealing ring, and a microfluidic network device () configured to supply reagents to the microfluidic chamber. The sampling device further comprises inlet and outlet distribution networks () in fluid communication with the microfluidic chamber and a slide holder () to guide and position said support containing a biological sample on the sampling device. The microfluidic network device comprises a plurality of reagent inlet channels () fluidly connectable to reagent sources, at least one reagent outlet channel () fluidly connected to the sampling device inlet distribution network (), and a plurality of valves () operable to selectively connect the inlet channels to the at least one outlet channel. The sampling device () and microfluidic network device () are formed on a common microfluidic support () as a single part.

Microfluidic cartridge with built-in sampling device

The invention relates to a method for in situ imaging of samples by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner. The invention is further related to imaging buffers preventing the degradation of the sample and of the imaging reagents, which are particularly useful in a method of in situ imaging of samples by cycle multiplexing according to the invention.

Methods of sample cycle multiplexing and in situ imaging

The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treatment of disease or disorders, in particular in the field of cancer management and therapy.

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Preverenges, Switzerland

Diego Gabriel Dupouy