Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Die Tang

Sanwen Huang

Chunzhi Zhang

Zhongmin Yang

Pei Wang

Yanhui Zhu

Provided herein are materials and methods for producing diploid, fertile, uniform, and vigorous hybrid potato. Also provided are methods of using advanced breeding methods, such as genome design, to generate potato inbred lines with high homozygosity which enables the exploitation of heterosis in this tuber crop and transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one.

Materials and methods for producing hybrid diploid potato

Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Sand Srespectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of SSfrom the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible Fgeneration by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

Method for breeding self-compatible potatoes

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Shenzhen, China

Die Tang