Université´ D'aix-Marseille

Centre National De La Recherche Scientifique

Institut National De La Sante Et De La Recherche Medicale

Biomerieux, Inc.

Assistance Publique Hopitaux De Marseille

Fondation Mediterranee Infection

Fondation Mediterranee Infection FacultÉ De MÉdecine

Protisvalor Mediterranee

Galderma Research & Development

Centre National De La Recherche Scientifique-cnrs

Inserm (institut National De La Sante Et De La Recherche Medicale)

Université De La Méditerranée - Aix-marseille II

Université De La Méditerranée

Universite De La Mediterranee (aix-marsille Ii)

Didier Raoult

Michel Drancourt

Bernard La Scola

Saber Khelaifia

Eric Chabriere

Mikael Elias

Pablo Gluschankof

Marie-Laure Birg

Florence Fenollar

Julien Hiblot

Jean-Marc Rolain

Najoua Ben M'Barek

Gilles Audoly

Claude Mabilat

Jean-Michel Brunel

Jérôme Aubert

Stéphanie Le Page

Christelle Perrin-East

Marion Bonnet

Sylvain Buffet

Nathalia Murillo

The present invention relates to a polyvalent culture medium for anaerobic bacteria under aerobic conditions in blood culture bottles. The present invention provides a polyvalent culture medium for culture in aerobic atmosphere of anaerobic bacteria or aerobic bacteria comprising a basal culture medium for bacteria characterized in that it further comprises a mixture of the following antioxidant compounds: sodium hydrosulphide (NaS), L-cysteine, ascorbic acid, glutathione, catalase, ubiquinol and lipoic acid.

Polyvalent culture medium for anaerobic bacteria under aerobic conditions

The present invention relates to a method of interpreting different antibiogram images in which it is possible to recognize a phenotype of bacterial resistance relative to antibiotics by comparing photographs using a photographic image bank of the reference antibiogram without any need to interpret them using the EUCAST or CA-SFM interpretation data; providing that for a given phenotype, there is available a collection of photographs of a plurality of bacteria of the same species and of the same phenotype.

Automated method for analyzing and interpreting an antimicrobial susceptibility

The present invention relates to a method for cultivating in vitro in an acellular culture medium, bacteria for which the growth is sensitive to oxygen content, said bacterium being selected from anaerobic bacteria and intracellular micro-aerophilic bacteria, characterized in that uric acid is added into said acellular culture medium, and said bacterium is cultivated in said culture medium in the presence of oxygen.

Use of uric acid for culturing bacteria sensitive to oxygen tension

Provided is a mutated hyperthermophilic PTE having a lactonase activity derived from a hyperthermophilic phosphotriesterase corresponding to the consensus sequence of SEQ ID NO: 1, the mutated PTE including the at least one mutation chosen amongst 55 putative positions and the mutated PTE having enhanced properties. Also provided are compositions including the mutated hyperthermophilic PTE and the uses thereof, notably as bioscavenger of organophosphate compounds or as quorum quencher of the bacteria using lactones to communicate.

Vulcanisaetal phosphotriesterase-like lactonases (PLL) having enhanced properties and the uses thereof

A method of characterizing specific microbiota associated withmites from a rosacea patient is described. The method can include a step of realizing a biopsy on the patient and analyzing the microbiota based on a method of 16SrRNA sequencing or pyrosequencing. A method for the diagnosis of rosacea in a patient is also described. This method can include characterizing microbiota associated withmites in the patient. Also described, are methods of differentiating a subtype of rosacea in a patient with rosacea and methods of controlling and measuring an active agent's treatment effectiveness on rosacea.

Method for the diagnosis of rosacea

Mutated hyperthermophilic PTE having a lactonase activity derived from a hyperthermophilic phosphotriesterase corresponding to the consensus sequence of SEQ ID NO: 1, the mutated PTE including the at least one mutation chosen amongst 53 putative positions and the mutated PTE having enhanced properties. Also provided are compositions including the mutated hyperthermophilic PTE and the uses thereof, notably as bioscavenger of organophosphate compounds or as quorum quencher of the bacteria using lactones to communicate.

Sulfolobal phosphotriesterase-like (PLL) lactonases activity having enhanced properties and the uses thereof

The present invention relates to the biological production of methane (Biogas) by co-culture in an aerobic atmosphere of a methanogenic bacterium and of an anaerobic bacterium capable of producing hydrogen, in a culture medium comprising or being supplemented with carbohydrate compound(s), notably starch and/or sugars, and supplemented with antioxidant compound(s).

Method for producing methane by means of aerobic co-culture of anaerobic micro-organisms

The present invention relates to the use of a compound chosen from squalamine and a squalamine-analog aminosteroid compound as agent for disinfecting an inert material object, in particular for the pre-disinfection of medical, dental, diagnostic or surgical equipment. The present invention also provides an aqueous or water-soluble disinfecting composition beneficial for a use according to the invention, characterized in that as disinfecting active compound it comprises a said antibacterial and antifungal compound selected from squalamine and a said squalamine-analog aminosteroid compound and suitable excipients for a water-soluble or aqueous formulation.

Use of squalamine or analogue as a disinfecting agent

A method of determining sensitivity or resistance of isolates of HIV retroviruses to a molecule includes a) amplifying sequences coding for a protease of a retrovirus to be studied, with or without the or some of amino acid sequences situated upstream and downstream of a cleavage site of a precursor in which the amino acid sequences are situated, b) recombining fragments of DNA, a final product of the amplification, and an expression vector allowing expression of sequence coding for the protease of the retrovirus to be studied under control of a known inducible promoter through co-transformation of the vector and the DNA fragments with at least one yeast cell, c) culturing co-transformed yeast cell or cells to obtain a sufficient number of transformants to perform a sensitivity or resistance test, and recovering transformants issuing from the co-transformed cell, on any suitable medium, d) incubating the transformants in the presence of a molecule to be tested, e) qualitatively or quantitatively analyzing the living cells, and f) deducing the sensitivity or resistance phenotype.

Yeast-based assay for measuring the functional activity of an HIV-1 protease in response to an antiviral agent

The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of thecomplex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of thecomplex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention. Finally, the present invention also relates to a method of identification in liquid phase by mass spectrometry which reduces time for diagnosing mycobacteriosis, particularly for tuberculosis.

Mycobacteria culture medium and method including mycobacteria of mycobacterium tuberculosis complex

RpoB gene sequences of various species ofbacteria, and a method of detection by molecular identification of various species ofbacteria using rpoB gene sequences.

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The invention relates to a method for in vitro seriological diagnosis of Whipple's disease, whereby the bacteria responsible for the disease are isolated and established in a culture and brought into contact with the serum or biological fluid of an infected patient. The invention also relates to useful oligonucleotides with a probe and a primer for amplifying, sequencing and detecting the gene rpoB of the bacteria,

Diagnosis of Whipple's disease

A method for determining sensitivity or resistance of isolates of HIV (human immunodeficiency virus) retroviruses to chemical molecules having an inhibiting activity on a viral protease or to therapeutic treatments based on inhibitors of the viral protease, including causing cell lysis of at least one yeast by expression of the retrovirus protease.

Methods for determining human immunodeficiency virus type 2 (HIV-2) protease sensitivity or resistance to antivirals utilizing an inducible yeast expression system

The invention relates to a method of detecting, by means of molecular identification, a bacterium from one of the-type species. The inventive method is characterised in that the following are used: a fragment of the rpoB gene of said bacterium, comprising a nucleotide sequence selected from one of the SEQ. ID. No. 11 to 39 sequences, the reverse sequences and the complementary sequences; or an oligonucleotide comprising a sequence having at least 12 consecutive nucleotide patterns included in one of the SEQ. ID. No. 7 to 10 sequences, in which N represents a nucleotide selected from inosine and an equimolar mixture of 4 different nucleotides selected from A, T, C or G and from the oligonucleotides of the reverse sequences and complementary sequences.

The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genusand four related generaandwhich consists of using as probe or primer:

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Marseilles, France

Didier Raoult