Abx S.a.

Horiba Abx Sas, Parc Euromedicine

Didier Lefevre

Henri Champseix

Serge Champseix

Roger Le Comte

Sylvie Veriac

Francis Lacombe

Francis Belloc

Ghislain Morel

Nadine Voltas

Bernard Kress

A subject of the invention is a method for discriminating between and counting at least two populations of biological elements carrying specific characteristics, optionally present in a sample. The invention allows the clear and unambiguous detection of at least three populations of biological elements by the use of only two detection means, which means that at least two populations of biological elements are detected by one and the same detection means. The invention can be carried out if three different probes are used, each recognizing and becoming fixed to one of the populations of biological elements to be detected, each of the probes being itself rendered detectable by a different marker, two of said markers having two emission spectra having at least one common part (overlapping emission spectra) and the third having an emission spectrum having essentially no part in common with the other two (non-overlapping spectrum).

Method of discriminating at least two cell populations, and application

The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.

Reagent and process for the identification and counting of biological cells

A device for automatic preparation of blood smears on plates comprises a storage unit for stacked new plates, an extractor unit to extract a plate from the stack, a depositing unit. To deposit a drop of blood on a plate, a spreading unit, to spread a drop of blood and produce a blood smear, a marking unit, comprising a marking head which can place means of identification on a reserved area of the plate, a drying unit, which can dry the blood smear, a coloring unit), which can color the blood smear, an output unit to unload the plate, and transfer means, to displace the plate between the aforementioned units, according to a pre-determined path.

Device for automatic preparation of blood smears on plates

It includes a measuring space (8) comprising a constrained passage (15) for the fluid, a monochromatic light source (31) delivering a beam (F1) to optical elements (6, 32, 34) including a first diffractive optical element (32) having a diffractive network (38) predetermined as a function, on the one hand, of the characteristics of the source and, on the other hand, of chosen geometrical and spectral characteristics for the illumination of the constrained passage, and suitable for inducing fluorescence of at least part of the fluid, and first (19-20), second (34-36) and third (40) optoelectronic elements intended to collect, respectively, a transmitted beam, fluorescence and a diffracted beam, and suitable for delivering to an analysis unit (24) signals relating to the contents of the beams. At least one of the beams emanates from a second diffractive optical element (42) having a second diffractive optical network which is predetermined as a function of a frequential and geometrical transfer function of at least one of the optoelectronic elements.

Device for the optical inspection of a fluid, especially for

A device for agitating and taking samples of blood products from tubes which are closed by stoppers and grouped together in a rack. The device includes a rack support adapted for holding at least one rack, at least one rack held by the rack support which is capable of holding a plurality of tubes with each tube being held in a selected position, a sampling station from which a sample of blood product can be withdrawn from a tube, a gripping device which is capable of taking hold and withdrawing from the rack one of the tubes at a selected position, bringing the tube to the sampling station and back to the selected position without agitation and replacing the tube in its selected position, and a drive device for displacing the gripping device. The device of the invention further includes a device for agitating the rack support and the rack which is operable during operation of the device for bringing and a device for withdrawing.

Device for agitating and for taking samples of blood products from tubes

The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.

Reagent for use in automatic analyzers for distinguisher leukocyte

The invention relates to a reagent and a method of using the same for automatically determining the population of the basophilic leukocytes in the blood. The invention relates more particularly to a new formulation for a differential lysing reagent that lyses all the blood cells except for the basophils, particularly suitable for the measuring principle based on resistivity measurement, that is to say the concentrations of the constitutents and the pH of the solution are suitable for ensuring good electrical conductivity of the medium. The reagent according to the invention will find an application in apparatus for automatically determining blood formulae by means of resistivity variation measurements.

Reagent and method of using same for automatically counting basophilic

An apparatus is disclosed for counting and determining at least one leucocytic sub-population, comprising a case for injecting a flow of sample to be analyzed inside a measurement tank through which a light beam passes, comprising internal and external nozzles for sleeving the sample by means of a pressurized liquid. Electrodes for the resistivity measurements of the leucocytic sub-population are component elements of the injection case. Units for collecting and processing the signals delivered by the optical sensor and the electrodes are also provided.

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Saint Clement de Riviere, France

Didier Lefevre