Mycartis N.v.

Didier Falconnet

Nicolas Demierre

Raphael Tornay

Mathieu Gaillard

José Gil

Patrick Van Den Bogaard

David Rebetez

David Bernasconi

Jose De Gil

Robin Muller

Raphaël Tornay

José De Gil

A method for determining fluorescence values {&#x3c6;}of a set of I fluorescent microparticles {&#x3bc;P}of a multiplexed analysis, the microparticles being in a monolayer arrangement, includes acquiring a digital fluorescence image of the set of fluorescent microparticles {&#x3bc;P}; and computing, for each fluorescent microparticle &#x3bc;Pin the set of fluorescent microparticles {&#x3bc;P}, a fluorescence value &#x3c6;based only on pixels of the acquired image corresponding to said fluorescent microparticle &#x3bc;P. The method includes computing the fluorescence value &#x3c6;of the fluorescent microparticle &#x3bc;Pby correcting its first fluorescence &#x3c6;by a cross-talk fluorescence contribution &#x3c6;in the first fluorescence &#x3c6;from other fluorescent microparticles {&#x3bc;P}in the set of fluorescent microparticles {&#x3bc;P}.

Cross-talk correction in multiplexing analysis of biological sample

The present invention relates to a microcarrier comprising at least a detection surface for performing an assay, said detection surface comprising a first area being functionalized with a first functional group for detecting at least a chemical and/or biological interaction, said first area being designed for providing a first signal. The microcarrier is characterized in that the detection surface further comprises a second area being designed for providing a second signal different from the first signal, said second signal being emitted during the assay. Thus, information about the presence of the at least a chemical and/or biological interaction is provided by a comparison of the first signal and the second signal.

Heterogenous surface functionalization

A method for performing a chemical and/or a biological assay including the following successive steps of: a) providing an assay device with a microchannel having an inlet and an outlet and restricting means for restricting movement toward the outlet of microparticles introduced in the microchannel while letting a fluid to flow through the restricting means, b) introducing microparticles in the microchannel via the inlet, c) restricting the movement of said microparticles in the microchannel toward the outlet using the restricting means, d) flowing a fluid sample through the microchannel, and e) performing a biological and/or chemical read-out on each microparticle. The method also includes the steps of: f) moving the microparticles in the microchannel, and g) repeating successively the steps d) and e).

Method and device for performing biological and/or chemical assays

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Vufflens-la-Ville, Switzerland

Didier Falconnet