Gen-probe, Inc.

Diane L McAllister

Daniel L Kacian

Nanibhushan Dattagupta

Sherrol Hoffa McDonough

Philip W Hammond

Thomas B Ryder

James John Hogan

Patricia C Gordon

Kathleen A Clark

A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

[object Object]

Hybridization assay probes are described which are able to distinguish Ureaplasma and known strains or serotypes of the species Ureaplasma urealyticum found in humans from other related organisms.

Nucleic acid probes to ureaplasma

Detecting Mycobacterium tuberculosis by nucleic acid sequence

A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reverse transcriptase and an RNA polymerase are provided to a mixture including the target sequence; the amplification proceeds accordingly. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

Oligonucleotide probes and methods for detecting Streptococcus pneumoniae

Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixure is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.

Method for suppressing inhibition of enzyme-mediated nucleic acid

Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.

Method for suppressing inhibition of enzyme-mediated reactions by ionic

Kits for suppressing inhibition of enzyme-mediated reactions by ionic

Methods of amplifying nucleic acids using promoter-containing primer

The present invention describes oligonucleotides targeted to Coccidioides immitis nucleic acid sequences which are particularly useful to aid in detecting Coccidioides immitis. The oligonucleotides can aid in detecting Coccidioides immitis in different ways such as by acting as hybridization assay probes, and/or amplification primers.

Growing community of inventors

San Diego, CA, United States of America

Diane L McAllister