University of Alberta

Neose Technologies, Inc.

Other

Biogenerix Ag

Diane E Taylor

Zhongming Ge

Monica Marija Palcic

Ge Wang

Karl Frank Johnson

Daniel James Bezila

Joanne Simala-Grant

David Rasko

Karl F Johnson

This invention provides nucleic acid and amino acid sequences of fucosyltransferases from. The invention also provides methods to use the fucosyltransferases to synthesize oligosaccharides, glycoproteins, and glycolipids.

Synthesis of oligosaccharides, glycolipids, and glycoproteins using bacterial glycosyltransferases

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A bacterial &#x3b1;1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &#x3b1;1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohy-drate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence ofin human specimens.

&#x3b1;1,3-fucosyltransferase

A bacterial &#x3b1;1,2-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &#x3b1;1,2-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, Lewis B and H type 1, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors.

&#x3b1;1,2 fucosyltransferase

A bacterial &#x3b1;1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &#x3b1;1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence ofin human specimens.

&#x3b1;-1,3 fucosyltransferases and expression systems for making and expressing them

A bacterial &agr;1,2-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &agr;1,2-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, Lewis B and H type 1, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors.

&agr;1,2-fucosyltransferase

A bacterial &agr;1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &agr;1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of in human specimens.

Growing community of inventors

Edmonton, Canada

Diane E Taylor