Assistance Publique-Hopitaux De Paris

Institut National De La Sante Et De La Recherche Medicale

Université´de Rouen

Chu De Rouen

Other

Centre National De La Recherche Scientifique

Biomerieux, Inc.

Université Paris Descartes

Université Paris Diderot-Paris 7

Universite Paris Cité

Université Montpellier 2 Sciences Et Techniques

Université Paris Diderot

Université Paris Nord

Diane Descamps

Christine Rouzioux

Francoise Brun-Vezinet

Jean-Noel Telles

Florence Damond

Marie Gueudin

Jean-Christophe Plantier

Valérie Courgnaud

Gilles Collin

Adeline Melard

Mélanie Bertine

Charles-Henri Lecellier

Véronique Avettand Fenoel

Véronique Avettand-Fenoel

Manuella Bouttier

The present invention relates to a method for detecting or quantifying deoxyribonucleic acid (DNA) of human immunodeficiency virus 2 (HIV-2) in a sample containing DNA comprising: a) performing a real-time polymerase chain reaction (PCR) on the sample, or a fraction thereof comprising DNA, with at least two sets of primers and probe each respectively comprising two primers and a labeled probe for the detection or quantification of HIV-2 DNA, at least one of the sets is selected from the group consisting of: a set comprising a primer comprising or consisting of a sequence SEQ ID NO: 1 or a sequence having al least 90% identity to SEQ ID NO: 1, a primer comprising or consisting of a sequence SEQ ID NO: 2 or a sequence having 90% identity to SEQ ID NO: 2 or the complement of these sequences, and a labeled probe comprising or consisting of a sequence SEQ ID NO: 3, or a sequence having at least 90% identify to SEQ ID NO: 3 or the complement of these sequences, and a set comprising a primer comprising or consisting of a sequence SEQ ID NO: 4 or a sequence having at least 90% identity to SEQ ID NO: 4, a primer comprising or consisting of a sequence SEQ ID NO: 5 or a sequence having 90% identity to SEQ ID NO: 5 or the complement of these sequences, and a labeled probe comprising or consisting of a sequence SEQ ID NO: 6 or a sequence having at least 90% identity to SEQ ID NO: 6 or the complement of these sequences, and b) determining therefrom the presence or absence and/or the quantity of HIV-2 DNA in the biological sample.

In vitro method for detecting and quantifying HIV-2 DNA

The present invention relates to a method for detecting or quantifying Human Immunodeficiency Virus-2 (HIV-2) nucleic acids in a biological sample, comprising: a) performing a real-time polymerase chain reaction (PCR) or a real-time reverse transcriptase polymerase chain reaction (RT-PCR) on nucleic acids of the biological sample with: (i) at least 4 primers respectively comprising or consisting of:

In vitro method for the detection and quantification of HIV-2

The invention relates to an in vitro method for identifying microRNAs or the target mRNAs thereof, the expression of which during the infection of cells by a virus using a cell receptor and at least one cell co-receptor for entering the cell, is specifically modified on the basis of the cell co-receptor used by the virus for its entering the cells, comprising:

Identifying viral cell tropism

A search method in a biological sample containing an HIV 2 viral strain for possible resistance of said strain to treatment by an anti-protease agent, and nucleotide probes for the implementation thereof. According to methods known per se, the presence of at least one mutation at certain, specified, particular positions of the proteinic sequence of the protease of said viral strain from a biological sample taken from a patient contaminated by HIV 2 is searched. If said mutation is observed, the existence of a resistance to said anti-protease agent is assumed in the patient.

Method for measuring resistance of a patient HIV-2 to protease inhibitors

Method for measuring anti-protease resistance of HIV-2 in a patient

Growing community of inventors

Paris, France

Diane Descamps