Dow Agrosciences LLC

Agrigenetics, Inc.

Kumar, Sandeep

Alabed, Diaa

Gupta, Manju

Diaa Alabed

Manju Gupta

Sandeep Kumar

Terry R Wright

Sarah E Worden

Todd P Glancy

Susan M Jayne

Kenneth Edwin Narva

Aaron Todd Woosley

Thomas Meade

Joel Jay Sheets

Stephanie L Burton

Doug A Smith

Sek Yee Tan

Karen C McLaughlin

Timothy Denver Hey

Sara Bennett

Nick X Wang

Carla Ausmus

Shao-Ching Hung

Xu Xiaoping

Karen C Mclaughlin

The subject invention relates in part to the surprising discovery that CryDa is active against corn earworm (CEW),(Boddie). Methods for using CryDa in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric CryDa demonstrated good insect protection against CEW larvae damage.

Cry1D for controlling corn earworm

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW),(Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.

Engineered and modified Cry6Aa insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Engineered Cry6A insecticidal proteins

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentspecies,v1.

Maize ubiquitin promoters

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentgenotype,c.v. Hi-II.

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentgenotype,c.v. B104.

CRY1D for controlling corn earworm

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentspecies,v2.

Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3'-UTRs, isolated fromubiquitin genes.

Constructs for expressing transgenes using regulatory elements from brachypodium ubiquitin 1C genes

Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3' untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

Use of a maize untranslated region for transgene expression in plants

Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from aUbiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Method and construct for synthetic bidirectional SCBV plant promoter

Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from aUbiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

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Carmel, IN, United States of America

Diaa Alabed