National Technology & Engineering Solutions of Sandia, LLC

Di Liu

John Michael Gladden

Gina Marie Geiselman

Samuel Coradetti

HeeJin Hwang

Peter Britton Otoupal

Methods and compositions (e.g., engineered hosts) are disclosed for use in converting biomass to 3-hydropropionic acid. In particular embodiments, the methods include use of an engineeredyeast, such as, the engineeredhaving the RT04_8975 gene deleted from its genome, combined with a lignocellulosic hydrolysate, sourced, for example, from a biomass. A promoter for enhancing transport of 3HP is also incorporated by addition to thegenome, for example, by modified lithium acetate transformation.

Modified yeast microorganisms to increase yield of 3-hydropropionic acid

The present disclosure relates to genetically engineered host cells and methods of producing a lipid-derived compound by employing such host cells. In particular embodiments, the host cell includes a first mutant gene encoding a cytoplasmic tRNA thiolation protein. Optionally, the host cell can include other mutant genes for decreasing fatty alcohol catabolism, decreasing re-importation of secreted fatty alcohol, or displaying other useful characteristics, as described herein.

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Emeryville, CA, United States of America

Di Liu