Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Dhruti Vasudev Dalal

Aruna Ayer

Christos Tzitzilonis

Corissa Harris

Matthew Dipietro

Liv Elisabeth Jensen

Charlotte Yang

Preethi Sarvabhowman

Tim Craig

Alexander Yang

Arkadiusz Bibillo

Cynthia Ann Cech

Eileen Thai

Ilya Lederman

Cynthia Cech

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

OmpG variants

OMPG variants

Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

Exonuclease deficient polymerases

The present of disclosure provides variant Pol6 polymerase polypeptide, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.

Polymerase variants

Growing community of inventors

Sunnyvale, CA, United States of America

Dhruti Vasudev Dalal