Mauk Et Al.

Dharma Teja Varapula

Sirisha Phanithra Gutta

Michael G Mauk

Methods that integrates processing and an analysis of a sample are disclosed and include use of a testing device that integrates nucleic acid isolation, isothermal amplification and detection wherein a sample and reagents are transported through a capture/binding membrane by capillary forces and the positioning of a slidebar that supports said binding membrane. In the practice of the methods, flow control and fluid actuation are effected by the position of a slidebar of the device which makes a fluidic connection through the capture membrane situated between a reservoir of liquid on the device and an absorbing pad, and where a sequence of operations for sample loading, washing, optionally amplification, and optical detection of analytes can be done conveniently and rapidly at the point of care.

Point-of-care diagnostic methods using capillary-action microfluidic devices

A testing device that integrates nucleic acid isolation, isothermal amplification and detection wherein a sample and reagents are transported through a capture/binding membrane by capillary forces and the positioning of a slidebar that supports said binding membrane. Flow control and fluid actuation are effected by the position of said slidebar which makes a fluidic connection through the capture membrane situated between a reservoir of liquid on the device and an absorbing pad, and where a sequence of operations for sample loading, washing, optionally amplification, and optical detection of analytes can be done conveniently and rapidly at the point of care. With minimal operator interaction, this device can lyse, isolate, amplify, and detect nucleic acids from clinical specimens and environmental samples.

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Philadelphia, PA, United States of America

Dharma Teja Varapula