Council of Scientific and Industrial Research

Other

Dharam Chand Jain

Sushil Kumar

Rajendra Singh Bhakuni

Suman Preet Singh Khanuja

Ram Prakash Sharma

Ajit Kumar Shasany

Mahendra Pandurang Darokar

Madan Mohan Gupta

Atul Prakash Kahol

Shiv Kumar Gupta

Guru Prakash Dutta

Dharmendra Saikia

Asish Kumar Bhattacharya

Suchi Srivastava

Ram Kishor Verma

Sudeep Tandon

Sudhanshu Saxena

Ram Asrey Vishwakarma

Tiruppadiripuliyur Ranganathan Santha Kumar

Satyawan Singh

Anil Kumar Dwivedi

Rajender Singh Sangwan

Digvijay Singh

Amit Tewari

Suman Preet Khanuja

Anita Gangwar

Ajay Kumar Mathur

Archana Mathur

Govind Ram

Aseem Umesh

Nuzhat Kamal

Tiruppadiripuliyur Ranganathan Kumar

Mohammed Shafique Siddique

Neerja Pant

Ranjendra Singh Bhakuni

Renu Tripathi

Sangeeta Dhawan

The present invention relates to a process for the isolation of artemisinin, an antimalarial agent from the herb of the plant, comprising of extracting the herb with ethanol, partitioning of the extract between water and hexane, followed by evaporative crystallization of artemisinin from hexane phase to produce substantially pure artemisinin.

Process for isolating artemisinin from Artemisia annua

This invention is related to the development of strategic and novel composition comprising &agr; arteether and nalidixic acid or quinolone drugs, said composition useful as an advanced generation drug to counter the resistance development itself and having a potential to be used in treating infectious diseases and in inhibiting the resistance developed due to mutation in the gyr A gene of bacteria, particularly in those cases where drug resistant strains are known to appear very frequently.

Anti-microbial composition and method for producing the same

The invention provides a method for maximization of artemisinin yield of the plant , said method comprising sowing seeds of plant on raised bed nursery during second and third week of December and maintaining the moisture throughout; transplanting seedlings thus obtained bearing at least 5-15 leaves into the main field fertilized with fertilizer, preferably NPK &commat; 80,40,40 kg/ha to achieve a population density of 50,000 to 200,000 per ha followed by light irrigation in the second week of March and irrigation every fortnight thereafter; harvesting the crop four times by cutting the plant tops leaving 75-100 cm part of plant for further regeneration, the said harvests are performed in a manner that the first harvest is done in fourth week of May, second harvest in third week of July, third harvest in second week of September and fourth harvest in third week of October of each year; and at each harvesting time care is taken to care at least one green branch, and extracting artemisinin from the plant tissue immediately after each harvest.

Method for maximization of artemisinin production by the plant artemisia annua

The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.) from the callus in about 40-60 days under light conditions (continuous light), and (d) Characteristic DNA profile wherein the lanes 1,2,3,4,5,6,7,8,9,10,11 and 12 in each gel (upper gel for wild line and lower gel for anthocyanin producing line) represent the PCR amplified fragments produced by the respective template megabase genomic DNA with primers 5&prime; CTG ATG CAT C3&prime;, 3&prime;,5&prime; TGG TCA CTG A 3&prime;, 5&prime; AGG GGT CTT G 3&prime;,5&prime; GAA ACG GGT G 3&prime;,5&prime; AGG GGT CTT G 3&prime;,5&prime; GCG TAA CGC C 3&prime;,5&prime; CAG CAC CCA C 3&prime;,5&prime; GTT GCG ATC C 3&prime;,5&prime; CAG GCC CTT C 3&prime;,5&prime; CGC AGT ACT C 3&prime;,5&prime; GTC CTA CTC G 3&prime;,5&prime; CTA CAC AGG C 3&prime; and 5&prime; GTC CTT AGC G 3&prime;, respectively. Lane M shows the standard size markers of a ladder at 100 bp intervals from 1000 bp downwards.

Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin

The present invention relates to a synergistic formulation useful for the control of wide spectrum of malarial infections, which comprises a pharmaceutically effective amount of dihydroartemisinin and a vegetable oil.

Process for the preparation of a formulation of dihydroartemisinin for the control of wide spectrum of malaria

The invention relates to an improved process for the preparation of arteether. The process comprises dissolving dihydroartemisinin in dry ethanol, adding a solid acid catalyst with trialkylorthoformate in the reaction mixture, stirring the reaction mixture at room temperature, adding H O to the reaction mixture, extracting the reaction product with a non-polar organic solvent, and drying the solvent over anhydrous sodium sulphate and evaporating the solvent to obtain pure arteether.

Process for the preparation of arteethers from dihydroartemisinin

The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.

Process for the isolation of compound scopoletin useful as nitric oxide synthesis inhibitor

The present invention relates to a synergistic formulation comprising pharmaceutically effective amount of &agr;/&bgr; arteether and a neutralized refined vegetable oil suitable for administration by rectal route, method for the treatment of cerebral and comatose malaria and process for the preparation of the said formulation.

Synergistic anti-malarial formulation

Formulation of dihydroartemisinin for the control of wide spectrum of malaria

The present invention relates to use .alpha.-arteether as a antibacterial agent, preferably for gyr mutant bacteria which are resistant to quinolone drugs and can be used as therapeutic agents for treating drug resistant bacterial infections, and also as a antifungal agent.

Method for the use of alpha arteether as an anti-bacterial and

This invention relates to a novel process for the dual production of essential oil and artemisinin from the plant Artemisia annua, said process comprising extracting the plant with hexane, partitioning the hexane extract between hexane and acetonitrile, hydrodistillation of hexane residue and Marc to yield essential oil, further fractionation of acetonitrile solvent between hexane-benzene mixture to remove artemisinic acid, chromatographing of the acetonitrile phase to produce substantially pure artemisinin, the artemisinic acid is reduced to obtain dihydro artemisinic acid which is then oxidised in a single step to form artemisinin.

Process for the simultaneous production of artemisnin and essential oil

Growing community of inventors

Lucknow, India

Dharam Chand Jain