Temasek Life Sciences Laboratory Limited

Deyu Xie

Yan Hong

The present invention is directed to a method ofregeneration through organogenesis and a method of genetic transformation of. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced toviainfection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.

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Singapore, Singapore

Deyu Xie