Becton Dickinson and Company Limited

Devon Joseph Jensen

Katherine Lazaruk

Dennis E Prosen

Eleen Shum

Christina Chang

James Ghadiali

Margaret Nakamoto

David Rosenfeld

Janice H Lai

Kai Liu

Ricelle A Acob

Cynthia Sakofsky

Disclosed herein include systems, methods, compositions, and kits for determining the expression of highly expressed proteins and lowly expressed proteins. In some embodiments, primers allowing generation of separate libraries for abundant AbSeq protein profiling oligonucleotides and scarce AbSeq protein profiling oligonucleotides are provided.

Profiling of highly expressed and lowly expressed proteins

Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

Oligonucleotides associated with antibodies

Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.

Primers for immune repertoire profiling

Growing community of inventors

San Jose, CA, United States of America

Devon Joseph Jensen