1859, Inc.

Omniome, Inc.

Pacific Biosciences of California, Inc.

Devon Cayer

Sean Stromberg

Pavel Chubukov

Andrew MacConnell

Ramesh Ramji

Eugene Tu

Kandaswamy Vijayan

Kerry Wilson

Corey M Dambacher

Joseph Franklin Rokicki

Richard LeCoultre

Richard Lecoultre

Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.

Methods and systems for microfluidic screening

Method of identifying a cognate nucleotide (i.e., the 'next correct nucleotide') for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide.

Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides

Growing community of inventors

Del Mar, CA, United States of America

Devon Cayer