Koninklijke Philips Corporation N.v.

Derk J W Klunder

Dirk Jan Broer

Stein Kuiper

Emiel Peeters

Anke Pierik

Hendrik Roelof Stapert

Bernardus H W Hendriks

Marius Iosif Boamfa

Maarten M J W Van Herpen

Maarten Van Herpen

Henk Van Houten

Maarten M J W Herpen

Maarten MJW Van Herpen

Richard J M Schroeders

A luminescence sensor, comprising a non-transparent substrate structure () having at least one aperture () intended to comprise an analyte and a transparent substrate structure (), which is arranged to or adjacent said first structure (). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench () with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.

Aperture biosensor with trenches

A device for monitoring radiation emitted by luminophores present in an analyte fluid of a wiregrid biosensor. The monitoring device comprises a non-polarized light source () for illuminating the wiregrid biosensor for exciting fluorescent labels arranged in the analyte fluid of said biosensor. A detector () detects radiation emitted by the labels after excitation. A polarizing filter () is arranged in between the transparent substrate and the detector for suppression of background emission radiation from labels positioned outside apertures in the wiregrid, in the analyte fluid.

Wiregrid monitor device

An optical device provides evanescent radiation, in response to incident radiation, in a detection volume for containing a target component in a medium. The detection volume has at least one in-plane dimension (W) smaller than a diffraction limit. The diffraction limit is defined by the radiation wavelength and the medium. The evanescent radiation is provided by aperture defining structures having a smallest in plane aperture dimension (W) smaller than the diffraction limit. The detection volume is provided between the aperture defining structures. The aperture defining structures further define a largest in plane aperture dimension (W). The largest in plane aperture dimension is larger than the diffraction limit. A source is provided for emitting a beam of radiation having a wavelength incident at the optical device and having a direction of incidence non parallel to an out of plane normal direction, for providing the evanescent radiation in the detection volume, in response to the radiation incident at the optical device. The plane of incidence may be parallel to the largest in plane aperture dimension.

Microelectronic sensor device

A method and sensor for the detection of luminescence radiation generated by at least one luminophore is disclosed. In the context of the present invention the biosensor comprises a grid () defined as arrays of apertures with the apertures having a first dimension below and a second dimension above the diffraction limit of the excitation light () in a medium, a polarizer (), and luminophores () positioned in a volume selected from the group consisting of: the volume inside the apertures of the grid (), the volume in between the array of slits () and the polarizer () and a volume that extends into the polarizer (), wherein the grid () providing a transmission axis extending in a first direction and the polarizer () providing a transmission axis extending in a second direction, the first direction and the second direction being substantially perpendicular with respect to each other, wherein the excitation radiation () is polarized such that it is substantially suppressed by one of the at least one grid () and polarizer () and substantially not suppressed by the other of the at least one grid () and polarizer ().

Biosensor with one-dimensional sub-diffraction-limited apertures composed of a grid and a polarizer

There is provided a method of detecting a presence of a luminophore in a detection volume comprising providing excitation radiation in said detection volume. A luminophore is provided in said detection volume being excitable by said excitation radiation. The luminescent radiation is detected to identify the presence of said luminophore in said detection volume. In one aspect of the invention, said luminophore is selected to emit luminescent radiation having a wavelength in said medium that is larger than twice said smallest dimension; and wherein said luminophore is selected to be excitable by excitation radiation having a wavelength in said medium that is smaller than twice said smallest dimension. Accordingly, luminescent radiation is blocked from entering the detector but for the portion present on an interface of the aperture.

Sub wavelength aperture

An optical waveguide comprises a body (), the body including an entrance window () and an exit window () defining an optical path () through a cavity. The cavity contains a first fluid (A) and a second fluid (B), with an interface between the first fluid and the second fluid defined by a meniscus. The meniscus lies longitudinally along the optical path. Means for adjusting the meniscus are provided, for example a voltage source and at least two electrodes. Electrowetting can be used for influencing the fluids.

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Eindhoven, Netherlands

Derk J W Klunder