Boston University

Nanoview Biosciences, Inc.

Derin Sevenler

M Selim Ünlü

Oguzhan Avci

George Daaboul

Celalettin Yurdakul

Selim M Unlu

David S Freedman

Steven Scherr

Jacob Trueb

Herein is described kinetic assay, in which individual binding events are detected and monitored during sample incubation. This method uses interferometric reflectance imaging to detect thousands of individual binding events across a multiplex solid phase sensor with a large area. A dynamic tracking procedure is used to measure the duration of each event. From this, the total rates of binding and de-binding as well as the distribution of binding event durations are determined. Systems and components for performing the kinetic assay are also described.

Dynamic tracking of captured targets for enhanced digital biosensing

An enhanced single particle interferometric reflectance imaging system includes an illumination source configured to produce illumination light along an illumination path toward a target substrate. The target substrate can be configured to reflect the illuminating light along one or more collection paths toward one or more imaging sensors. The target substrate includes a base substrate having a first reflecting surface and a transparent spacer layer having a first surface in contact with the first reflecting surface and a second reflecting surface on a side opposite to the first surface. The transparent spacer layer has a predefined thickness that is determined as a function of a wavelength of the illuminating light and produces a predefined radiation pattern of optical scattering when nanoparticles are positioned on or near the second reflective surface. In addition, one or more of the collection paths can also include an amplitude mask selected to match the radiation pattern.

Method and system for enhanced single particle reflectance imaging

A system for analyzing one or more liquid samples includes a microwell plate including a plurality of rows of wells configured to store liquid samples, a sensor array that is moveable relative to the microwell plate along a first axis between a first position and a second position to allow a portion of the sensor array to be disposed within a first one of the plurality of rows of wells when the sensor array is in the second position, an objective, and one or more linear translation stages configured to move the microwell plate relative to the objective (i) along a second axis that is orthogonal to the first axis, (ii) along a third axis that is orthogonal to the first axis and the second axis, or (iii) both (i) and (ii).

Systems and methods for imaging microwell plate samples

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Derin Sevenler