Element Biosciences, Inc.

Sanford-burnham Medical Research Institute

Burnham Institute for Medical Research

Vala Sciences, Inc.

Echo Laboratories, Inc.

Derek Fuller

Michael Previte

Chunhong Zhou

Minghao Guo

Steve Xiangling Chen

Jeffrey H Price

Albert L Kellner

Giacomo Strollo

Eugene L Cho

Behrad Azimi

Dorian Raymer

Anthony Beatty

Adam Rusch

Martin Weinigel

Steven Xiangling Chen

Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

High performance fluorescence imaging module for genomic testing assay

Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.

Tube lens design for improved depth-of-field

Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.

Nucleic acid sequencing systems

Multifunction autofocus for automated microscopy includes automatic coarse focusing of an automated microscope by reflective positioning, followed by automatic image-based autofocusing of the automated microscope performed in reference to a coarse focus position. In some aspects, the image-based autofocusing utilizes astigmatism in microscope optics for multi-planar image acquisition along a Z axis direction of a microscope. In some other aspects, the image-based autofocusing utilizes astigmatism in microscope optics in combination with chromatic aberration for multi-planar image acquisition along the Z axis direction.

Multifunction autofocus system and method for automated microscopy

Aspects of the subject technology provide a fluorescence module that is configured to provide a selectable light source for fluorescence microscopy, e.g., through independent paired selection of a light source (emitter) and a corresponding light filter. In some implementations, light source and/or light filter selection is controlled through the independent actuation of light sources and/or light filters on a supporting turret structure.

Fluorescence module with a plurality of filters and light sources

In an automated scanning cytometry system, chromatic aberration is used for multiplanar image acquisition.

Automated scanning cytometry using chromatic aberration for multiplanar image acquisition

Growing community of inventors

San Diego, CA, United States of America

Derek Fuller