Viterra Inc.

Other

Saskatchewan Wheat Poo1

Saskatchewan Wheat Pool

Basf Se Corporation

Derek Arthur Potts

Daryl Richard Males

Kening Yao

Bradley D Leibel

Gerhard Friedrich Werner Rakow

John Philip Raney

David Charles Taylor

Katherine M Lockhart

Laurie Friesen

Roberto Bacchetto

Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of theB genome. TwoAHAS gene sequences (BjAHAS-a and BjAHAS-b) and oneAHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutantlines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.

[object Object]

In various aspects, the invention providesplants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than otherplants. In one embodiment, theline MJ02-357-3 contains a mutant allele MJ02-313-1/BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a G to A substitution in the ORF at position 281 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Glycine-94 Aspartic acid mutation in the sequence of the predicted BjFAD2-a protein. In another embodiment, theline MJ02-357-3 contains a mutant allele MJ02-357-3/BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a C to T substitution in the ORF at position 647 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Proline-216 Leucine mutation in the sequence of the predicted BjFAD2-a protein. As a result of these mutations, it can be predicted that the function of the BjFAD2-a proteins are negatively affected inlines MJ02-313-1 and MJ357-3 as reflected in the increased levels of oleic acid in seed oil in comparison with the wild-type line J96D-4830. Seeds from MJ02-313-1 and MJ02-357-3 plants may for example yield an oil having oleic acid content of greater than 70% by weight.

In one aspect, the invention provides novel tetraploidplants having no more than two expressible FAD2 coding sequences, capable of producing canola quality oils. Other aspects of the invention provide new variants of the FAD2 enzyme, comprising BjFAD2-b and BjFAD2-a, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include nucleic acid sequences upstream from the BjFAD2-b or BjFAD2-a ORFs. Other aspects of the invention include transgenic plants and plant pads. Vectors capable of transforming plant cells are provided, comprising the nucleic acids of the invention, including FAD2 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products. Amplification primers for identifying the FAD2 coding sequences of the invention are provided, together with methods of obtaining plants using the FAD2 coding sequences of the invention as markers.

Plant FAD2 coding sequence balancing for fatty acid profiling in edible oils

In various aspects, the invention providesplants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than otherplants. In one embodiment, theline MJ02-086-3 contains a mutant allele MJ02-086-3/BjFAD2-a at the BjFAD2-a gene locus, having a premature stop codon, so that there are no functional copies of the FAD2 gene in MJ02-086-3 plants. Seeds from MJ02-086-3 plants may for example yield an oil having oleic acid content of greater than about 70% by weight.

Novel lines of yield an edible oil that has properties similar to canola oil. More specifically, the oil has an oleic acid content of at least 55% by weight, a linoleic acid content of less than 25% by weight, a linolenic acid content of less than 14% by weight, a erucic acid content of less than 1% by weight, a palmitic acid content of less than 6% by weight, a stearic acid content of less than 2.5% by weight, and a total saturated acid content of less than 7.1% by weight. The novel lines of plant have a lineage that includes both lines J90-3450 and J90-4316, these being known lines of , and may be formed by conventional cross fertilization or other means. The novel lines can be grown in harsher environments than conventional canola species and are high-yielding and disease-resistant.

Brassica juncea lines bearing endogenous edible oils

In various embodiments, the invention provides for the use of an ADS1 or ADS2 &Dgr;9 fatty acid desaturase to selectively increase the relative proportion of oleic acid in the fatty acid of a plant part, such as in the oil of a mature seed. In some embodiments, the proportion of oleic acid may be increased preferentially, without a corresponding or proportional increase in palmitoleic acid.

Selective modification of plant fatty acids

Novel lines of,yield an edible oil that has properties similar to canola oil. More specifically, the oil has an oleic acid content of at least 55% by weight, a linoleic acid content of less than 25% by weight, a linolenic acid content of less than 14% by weight, a erucic acid content of less than 1% by weight, a palmitic acid content of less than 6% by weight, a stearic acid content of less than 2.5% by weight, and a total saturated acid content of less than 7.1% by weight. The novel lines of,plant have a lineage that includes both lines J90-3450 and J90-4316, these being known lines of,and may be formed by conventional cross fertilization or other means. The novel lines can be grown in harsher environments than conventional canola species and are high-yielding and disease-resistant.

Growing community of inventors

Saskatoon, Canada

Derek Arthur Potts