Life Technologies Corporation

Other

Applied Biosystems, LLC

Life Technologies Coporation

Hennessy, Lori

Dennis Wang

Lori Hennessy

Chien-Wei Chang

Robert L Green

Julio Mulero

Robert Lagace

Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

SE33 mutations impacting genotype concordance

Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

Fast PCR for STR genotyping

Boat rack

Supporter for watercraft

The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.

Method for direct amplification from crude nucleic acid samples

A clamping device includes a holding member including a space for allowing an item to be disposed therein, and a tightening mechanism operable to make the holding member clamp the item in the holding member. The space is varied in size depending upon the external diameter of the clamped item. Additionally, the tightening mechanism is provided for enabling a satisfactory securing condition for any size of items.

Clamping device

Growing community of inventors

Dublin, CA, United States of America

Dennis Wang