Gradipore Limited

The Texas A&m University System

Dennis Rylatt

David Ogle

Chenicheri Hariharan Nair

Gyula Vigh

Andrew Mark Gilbert

Brendon Francis Conlan

Lucy Jane Ryan

Theresa Marie Thomas

An electrophoresis apparatus for processing compounds in small sample volumes comprising a cathode in a static cathode buffer zone or compartment, an anode in a static anode buffer zone or compartment. The cathode disposed relative to the anode so as to be adapted to generate an electric field in an electric field area therebetween upon application of a voltage potential between the cathode and anode. A removable cartridge disposed in the electric field area between the anode and the cathode. The cartridge containing a first non-isoelectric separation barrier and also a second non-isoelectric separation barrier disposed between a selected one of the cathode buffer zone and the anode buffer zone and the first barrier so as to define a first chamber having an interstitial volume of less than 5 ml therebetween.

Small separation apparatus

An electrophoretic apparatus comprising: a first electrolyte chamber containing a first electrode; a second electrolyte chamber containing a second electrode; a first sample chamber disposed between the first and second electrolyte chambers and proximate to the first electrolyte chamber; a second sample chamber disposed between the first sample chamber and the second electrolyte; three ion-permeable barriers separating the first electrolyte chamber, the first sample chamber, the second sample chamber, and the second electrolyte chamber, respectively, wherein the ion-permeable barriers impede convective mixing of the contents in each of the respective chambers; a first electrolyte reservoir and a second electrolyte reservoir in fluid communication with the first and second electrolyte chambers, respectively; a first sample reservoir and a second sample reservoir in fluid communication with the first and second sample chambers, respectively; means adapted for communicating a first electrolyte and a second electrolyte between the respective electrolyte chambers and reservoirs; means adapted for communicating a first fluid and a second fluid between the respective sample chambers and reservoirs, wherein at least one of the first and second fluid contains at least a sample, wherein application of an electric potential causes migration of at least one component through at least one of the ion-permeable barriers.

Electrophoresis apparatus and method

A system for separating biomolecules by electrophoretic separation including a first separation barrier having a defined molecular mass cut-off disposed in the first electric field area, a first restriction barrier disposed between a first cathode zone and a first separation barrier so as to define a first interstitial volume therebetween, a second restriction barrier disposed between the first anode zone and the first separation barrier so as to define a second interstitial volume therebetween, and a pumping means to provide a sample constituent in a selected one of the first interstitial and second interstitial volumes wherein upon application of the first voltage potential, a selected separation product is removed from the sample constituent through the first separation barrier and provided to the other of the first and second interstitial volumes.

Electrophoresis separation and treatment of samples

Growing community of inventors

Ryde, Australia

Dennis Rylatt