Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Dennis O'Neill

Carl R Simmons

Haiyin Wang

Dale F Loussaert

Igor Cunha De Oliveira

Jijun Zou

Andrew Slattery

The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (QPCR or real-time PCR), wherein the real-time PCR is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.

Method for high-throughput screening of transgenic plants

The NR enzymes described herein were discovered in the red algae of(PpNR) and(PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

Nitrate reductases from red algae, compositions and methods of use thereof

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Ankeny, IA, United States of America

Dennis O'Neill