University of California

University of Southern California

California Institute of Technology

Other

Dennis O Clegg

David R Hinton

Lincoln Vallance Johnson

Sherry T Hikita

Mark Salman Humayun

Robert H Grubbs

Yu-Chong Tai

Ashish Ahuja

Mark S Humayun

Liane Miller

Marlene Tsie

Chelsea D Presbrey

Lisa R Conti

Britney O Pennington

Michelle A Maloney

Qirui Hu

Danhong Zhu

Peter Coffey

Lyndsay Leach

Jeffrey K Bailey

Cassidy Arnold

Mohamed A Faynus

Debbie Mitra

David Buchholz

Roxanne Croze

Robert Grubbs

Britney Pennington

Disclosed herein are methods and compositions for the identification of viability enhancing cell features and substrate features as they relate to post-cryopreservation survival of substrate seeded cells. Embodiments of the present invention further involve identification of cell features to manufacture a supernatant that is useful for cell culturing and treatment of various diseases.

Cryopreservation of cell-seeded substrates and related methods

Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for  implanting same

The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

Methods of culturing retinal pigmented epithelium cells, including Xeno-free production, RPE enrichment, and cryopreservation

Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same

Methods of culturing retinal pigmented epithelium cells, including xeno-free production, RPE enrichment, and cryopreservation

The invention relates to the field of cell culture, specifically, the derivation of retinal pigmented epithelial cells from pluripotent cells. The invention comprises the use of various cell culture medium supplements, medium formulations, and methods of using such medium supplements and formulations, in order to effect the rapid differentiation of pluripotent cells into retinal pigmented epithelial cells with very high yields. The invention further includes cell culture media formulations for the efficient maintenance, propagation, and maturation of cultured retinal pigmented epithelial cells.

Methods and compositions for the rapid production of retinal pigmented epithelial cells from pluripotent cells

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Ventura, CA, United States of America

Dennis O Clegg