Diversa Corporation

Hercules Corporation

Verenium Corporation

Diverso Corporation

Dennis Murphy

John H Reid

Dan Eric Robertson

Jay Milton Short

Eric J Mathur

Ronald V Swanson

Anthony M Maffia, Iii

Steven Link

Anna Kosmotka

Patrick Vernon Warren

Lei Qiao

Qu-Ming Gu

Huai Nan Cheng

William Joseph Coleman

Edward J Bylina

Wenhua Xie

Peng George Wang

Simon Delagrave

Jennifer L Rittenhouse Pruss

Anthony M Maffia

Patrick V Warren

The invention relates to esterases and to polynucleotides encoding the esterases. In addition methods of designing new esterase method of use thereof are also provided. The esterases have increased activity and stability at increased pH and temperature.

Nucleic acids encoding esterases and methods of making and using them

The invention relates to &#x3b1;-galactosidase and to polynucleotides encoding the &#x3b1;-galactosidase. In addition methods of designing new &#x3b1;-galactosidases and method of use thereof are also provided. The &#x3b1;-galactosidases have increased activity and stability at increased pH and temperature.

Assays for identifying &#x3b1;-galactosidases

&#x3b1;-galactosidases and methods for making and using them

A thermostable alpha-glycosidase derived from variousAEDII12RA is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry.

&#x3b1;galactosidases and methods of using them

Polysaccharides comprising one or more oxime linkages are provided. Methods for their preparation include the polycondensation of saccharides bearing oxime-forming substituents. In some embodiments, polymerization is conducted in the presence of galactose oxidase. The resulting oxime-linked polysaccharides have desirable properties and are useful in numerous applications including paper manufacturing and drug delivery vehicles.

Oxime-linked polysaccharides and methods of preparing the same

A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or 'natural' enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionality, a step which is very difficult to achieve with nonenzymatic methods.

Amidase

A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or 'natural' enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionally, a step which is very difficult to achieve with nonenzymatic methods.

The present invention provides mutant genes encoding variant galactose oxidase enzymes, termed vGOs; constructs and recombinant host cells incorporating the genes; the vGO polypeptides encoded by the genes; antibodies to the polypeptides; and methods of making and using all of the foregoing.

Variant galactose oxidase, nucleic acid encoding same, and methods of using same

A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or `natural` enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionality, a step which is very difficult to achieve with nonenzymatic methods.

AMIDASE

A thermostable alpha-glycosidase derived from various Thermococcus, alcaliphilus AEDII12RA is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry.

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Malvern, PA, United States of America

Dennis Murphy