Pioneer Hi-bred International, Inc.

Other

E.i. Dupont De Nemours and Company

J.r. Simplot Company

Dennis L Bidney

Christopher Jay Scelonge

William James Gordon-Kamm

Leszek Aleksander Lyznik

Yumin Tao

Igor Falak

David George Charne

Glenn S Cole

Mark K Mancl

Guihua Lu

Katherine A P Nazarian

Xu Hu

Keith S Lowe

Oswald R Crasta

Lijuan Wang

James Jefferson Smith

Derek Jantz

Michael D Miller

Huirong Gao

Sean J Coughlan

Craig E Hastings

Margit C Ross

Raymond J Taylor

Gary Allen Secor

Mike Lassner

Cheryl Louise Ruby

Saverio Carl Falco

Andrew Mark Cigan

Dwight T Tomes

Benjamin A Bowen

Gary A Huffman

Jonathan P Duvick

Jon Duvick

Zuo-Yu Zhao

George J Hoerster

Gary A Sandahl

Xinli Emily Wu

Dorothy A Pierce

Carol A Hendrick

Charisse M Buising

Laura A Church

Mark Cigan

Carl Falco

Katherine A Nazarian

Patrea M Hill

William Gordan-Kamm

Andre A Steinmetz

Rachel Yvonne Baltz

Evan Dale Elsing

Mike Miller

Gary Sandahl

Evan D Elsing

Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.

Modified FRT recombination site libraries and methods of use

Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

Methods and compositions for producing male sterile plants

Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

FRT recombination sites and methods of use

Methods for producing homozygous plants, seeds, and plant cells are provided. The methods comprise transforming a somatic cell of a maize haploid embryo with a polynucleotide of interest and treating the transformed cell with a chromosome doubling agent. One or more growth stimulation proteins, such as, for example, RepA or Lec1, may also be provided.

Methods of transforming somatic cells of maize haploid embryos

Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a LOX protein are provided. Nucleotide sequences comprising the LOX promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.

Sunflower LOX polynucleotides and related compositions

The invention relates to isolated sunflower nucleic acid sequences encoding a protein having antipathogenic activity, vectors, plant cells, plants and seeds comprising said nucleic acid sequences. The invention further relates to a method of transforming plants for increased resistance against plant pathogens.

Sunflower anti-pathogene proteins and genes and their uses

The invention relates to the genetic manipulation of plants, particularly to the expression of nucleotide sequences from defense-related signaling genes. Isolated nucleotide sequences encoding a neoxanthin cleavage enzyme, an amino acid permease, and a novel protein are provided. Also provided are isolated nucleotide sequences comprising promoters that drive expression in a plant in an inducible or tissue-preferred manner. The nucleotide sequences find use in increasing the resistance of plants to pathogens and other stresses, modifying ABA metabolism in plants, modifying amino acid transport and content in plants, and regulating gene expression in plants. Additionally provided are isolated proteins, and transformed plants and seeds thereof.

Defense-related signaling genes and methods of use

Compositions and methods for enhancing disease resistance in plants are provided. Compositions comprise sunflower resistance gene analogs. The methods involve transforming a plant with a resistance nucleotide sequence. The methods find use in enhancing broad-based resistance in plants to pathogens. Also provided are transformed plants, plant cells, tissues, and seeds having enhanced disease resistance. The R gene analogs can be used as molecular markers for disease resistance.

Sunflower disease resistance genes

The invention provides improved plant transformation methods. In particular the method provides increased transformation freequency, especially in recalcitrant plants. The method comprises stably transforming a target cell with at least one polynucleotide of interest. The target cell has been previously transformed to stimulate growth of the cell and has gone through at least one cell division.

Methods for enhancing plant transformation frequencies

Growing community of inventors

Urbandale, IA, United States of America

Dennis L Bidney