University of Florida Research Foundation Inc

Other

The United States of America As Represented by the Secretary of

Dennis J Gray

Zhijian T Li

Jayasankar Subramanian

Ralph Scorza

Richard E Litz

Zhijian Li

Gary W Elmstrom

The subject invention pertains to methods and materials for enhancing microbial resistance in plants. Specifically exemplified herein are grapevines transformed with polynucleotides that express a peptide which confers antimicrobial activity.

Antimicrobial peptides and uses thereof

This invention is a device for transforming the energy of water waves into useable energy. The device comprises two or more floats, structural members connecting the floats, a means for transferring torque, and one or more generators.

Energy transformation device

This device transforms the energy of flowing water into useable energy and includes one or more flow diversion structures, one or more paddlewheels, and one or more generators. A gearbox or transmission may be added to increase the rotational speed of the generators. A subsea cable can be included to transmit the electrical energy from device to shore.

Flowing water energy device

The invention features a method of selecting for a grape somatic embryo or grape plant having resistance to a plant pathogen using a liquid culture medium. The method including the steps of (a) culturing a grape embryonic culture in a first liquid culture medium to grow a cellular suspension culture, the first liquid culture medium including a plant growth regulator and a filtrate from a plant pathogen culture; (b) recovering an embryogenic cell or embryogenic cell mass from the cellular suspension culture; and (c) culturing the embryogenic cell or embryogenic cell mass in a second liquid culture and in a third culture media to select a grape somatic embryo or a grape plant having resistance to a plant pathogen.

Selection of fungal resistant grape somatic embryos

The subject invention pertains to the isolation of nucleotide sequences of the 2S albumin gene and its promoter from grape. Promoter sequences of the invention provide for seed-specific transcription of operably linked polynucleotide sequences. Seed-specific expression activity of the subject promoter was also characterized by using transient green fluorescent protein (GFP) expression analysis in transformed somatic embryos (SE) of grape (cv. Thompson Seedless). The subject invention also concerns expression constructs comprising a promoter of the present invention. Plants, plant tissues, and plant cells bred to contain or transformed with a polynucleotide of the invention are also contemplated by the present invention.

Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof

Disclosed are methods for producing grape plants having resistance to bunch rot disease oror both. Also disclosed are grape plants having resistance to bunch rot disease oror both, wherein the plants express a cecropin B peptide Shiva-1.

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The present invention is directed to bidirectional promoter complexes that are effective for enhancing transcriptional activity of transgenes. The bidirectional promoters of the invention include a modified enhancer region with at least two core promoters on either side of the modified enhancer in a divergent orientation.

Bi-directional dual promoter complex with enhanced promoter activity for transgene expression in eukaryotes

The invention features a method of producing a grape somatic embryo having resistance to a plant pathogen, the method including the steps of (a) culturing a grape somatic embryo in a first liquid culture medium that includes a plant growth regulator and a phytotoxin from a plant pathogen; (b) exchanging the first liquid culture medium for a second liquid culture medium not including the phytotoxin; (c) recovering a living grape cell or grape cell cluster from the second liquid culture, the living cell or cell cluster being resistant to the pathogen; and (d) culturing the grape cell or grape cell cluster in a third culture medium to produce a grape somatic embryo.

Pathogen-resistant grape plants

The invention features methods of producing a plant or a mature somatic embryo from an embryogenic cell or embryogenic culture. The method includes the steps of: (a) providing a liquid culture that includes an embryogenic cell; (b) recovering embryogenic cell from the culture; (c) transferring the embryogenic cell to a second culture; and (d) growing a mature somatic grape embryo from the embryogenic cell.

Regeneration system for grape and uses thereof

Disclosed are methods for producing transgenic grapevines with resistance to a plant pathogen, the method includes: transforming a plant cell of the genus Vitis with a nucleic acid which expresses a lytic peptide, where the expression of the lytic peptide provides resistance to a plant pathogen.

Disease resistance in vitis

Disclosed here is a novel process which facilitates the rapid and economical production of seedless watermelon seed. The novel process involves cloning desirable tetraploid watermelon parental lines. These parental lines are essential in the production of triploid seed for the seedless watermelon. The subject invention makes possible the use of self-sterile tetraploid parental lines in the production of triploid seed.

Growing community of inventors

Howey-in-the-Hills, FL, United States of America

Dennis J Gray