Becton Dickinson and Company Limited

Life Technologies Corporation

Bio-rad Laboratories, Inc.

Dennis E Prosen

Katherine Lazaruk

Lei Xi

Yan Wang

Stephen P Hendricks

Zhaochun Ma

Paul M Kenney

Devon Joseph Jensen

Kai Liu

Ricelle A Acob

Roland Nagel

Eleen Shum

Christina Chang

James Ghadiali

Janice H Lai

David Rosenfeld

Lei (Larry) Xi

Devon Jensen

Tracy Campbell

Ricelle Agbayani Acob

Xiaoshan Shi

Larry L Xi

Alexander A Novikov

Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.

Primers for immune repertoire profiling

Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

Oligonucleotides associated with antibodies

Disclosed herein include systems, methods, compositions, and kits for determining expressions of proteins and genes simultaneously, and for sample indexing. The method can comprise extending a cellular component-binding reagent specific oligonucleotide hybridized to an oligonucleotide barcode to generate an extended cellular component-binding reagent specific oligonucleotide. The extended cellular component-binding reagent specific oligonucleotide can be separated from the oligonucleotide barcodes. The separated extended cellular component-binding reagent specific oligonucleotide can be amplified separately from barcoded cDNA.

Methods and compositions for quantitation of proteins and RNA

This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or &#x3bc;-monothiopyrophosphate), and related compounds.

Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions

This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

Nucleic acid modifying enzymes

Growing community of inventors

San Jose, CA, United States of America

Dennis E Prosen