Agracetus

Monsanto Technology LLC

Powderject Vaccines, Inc.

Monsanto Company

Other

W. R. Grace Co.-conn.

W. R. Grace Co.

Cetus Corporation

Powederject Vaccines, Inc.

Dennis E McCabe

Brian J Martinell

Richard J Heinzen

Edward James Williams

Yong Huang

Paul Christou

Lori S Julson

Carol A Emler

Beth Jo Calabotta

Alan S Paau

Lori Ann Smith

Gail A Roberts

Richard F Klemm

William F Swain

Steven G Platt

Donald A Glaser

Kenneth A Barton

Brent H McCown

Dannette Connor-Ward

Joseph K Burkholder

Maud Ann Hinchee

Rodney A Serres

Lori L Graham-Weiss

Carl Emler

Lori A Julson

Brian Martinell

Beth J Calabotta

A method is disclosed for the-mediated germline genetic transformation of soybean. The method is based on-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.

Soybean transformation method

A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high-throughput of viable, transplantable tissue.

Method and apparatus for preparation of genetically transformable plant tissue

A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on an Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, wherein cells are induced directly to form shoots and give rise to transgenic plants. This method does not involve a callus-phase tissue culture and is rapid and efficient.

Soybean agrobacterium transformation method

Particles coated with a therapeutic agent are deposited onto the inner surface of a length of tubing using a process which includes introducing the particles into the tubing while the tubing is rotating horizontally. An apparatus for performing the process is also disclosed.

Apparatus for preparing sample cartridges for a particle acceleration device

A method and apparatus is disclosed for the genetic transformation of organisms by accelerated particle mediated transformation. Foreign genes are introduced into cells by coating on carrier particles which are physically accelerated into the cells. The treated cells are recovered, and a portion of them will contain in their genome the foreign gene. The procedure may be used to create genetically engineered organisms of many types.

Method for genetic transformation

A sample delivery module for use in conjunction with a particle acceleration apparatus includes a fitting for connecting the sample delivery module to the apparatus. The sample delivery module provides a particle acceleration path which includes a portion adapted to receive a sample of particles coated with a biological material, a particle acceleration chamber coaxial with, and in fluid communication, with the cartridge chamber, and a substantially conical exit nozzle coaxial with and in fluid communication with the particle acceleration chamber. In use, a gas stream causes sample particles to be entrained therein and accelerated through the particle acceleration chamber and the exit nozzle toward a target.

Sample delivery module for particle acceleration apparatus

A method is disclosed for making more efficient the particle-mediated germ line genetic transformation of bean species such as soybean. After a particle-mediated transformation event, in the absence of a selectable marker gene, relatively large numbers of plants must be regenerated to find the relatively low likelihood germ line transformation events which have occurred. It has been discovered that using in the transformation process a marker gene linked to the gene of interest, and by excising a segment of the stem of the shoot during the regeneration process and assaying the segment for the marker gene, certain patterns or phenotypes can be identified in the stem segment which are associated with an increased frequency of germ line transformation events. As the plants are regenerated, other indices of gene expression, at the first trifoliate leaf stage and at the third or fourth trifoliate leaf stage, also serve as markers of the likelihood of germ line transformation. By using these markers in the relatively early stages of plant regeneration to assay for likelihood of germ line events, it is possible to concentrate regeneration efforts on plants most likely to yield germ line events, and to discard the others, so as to lower the burden and effort in achieving a desired number of transformation events.

Plant transformation with early identification of germ line

A transformation protocol is described for the particle mediated transformation of soybean. This protocol is based on gene delivery into the growing meristem of a soybean embryo. Prior transformation protocols based on meristematic gene delivery in soybean did not depend on selection due to difficulties in using selection agents in differentiated tissue in soybean. It has been found that a post bombardment culture with glyphosate selection can dramatically increase the efficiency of such a transformation protocol.

Efficiency soybean transformation protocol

A gas driven apparatus for accelerating particles coated with a genetic material into a target comprises a reservoir for releasably retaining a gas at a sufficiently high pressure to detach the particles from the surface of a sample cartridge and to carry the particles through the apparatus toward the target. When leaving the apparatus, the particles entrained in the gas stream pass through a substantially conical exit nozzle which causes the pattern of distribution of the particles to greatly expand. Methods for using the apparatus and for preparing the sample cartridges are also described.

Gas driven gene delivery instrument

Plant transformation process with early identification of germ line

A method for depositing particles coated with biological substances onto the concave inner surface of a length of tubing includes the steps of preparing a uniform suspension of coated particles, introducing the particles into the tubing, placing the tubing into a generally horizontal position, removing the evaporable liquid after the particles have settled, and drying the particles. When dry, the tubing is cut into sample cartridges of appropriate length for use in a particle acceleration instrument. An apparatus for performing the method is also disclosed.

Method and apparatus for preparing sample cartridges for particle

Growing community of inventors

Middleton, WI, United States of America

Dennis E McCabe