Dupont Nutrition Biosciences Aps

Universite Laval (canada)

International N&h Denmark Aps

Dennis A Romero

Philippe Horvath

Christophe Fremaux

Rodolphe Barrangou

Patrick Boyaval

Sylvain Moineau

Alexander Hynes

Geneviève Rousseau

Joshua K Young

Simon Labrie

Anne M Millen

Phillippe Horvath

Marie-Laurence Lemay

Lindsay L Traeger

Armelle Cochu-blachère

Rodolph Barrangou

The present invention relates to a lactic acid bacterium, in particular a strain of thegenus, with reduced sensitivity to cos-type bacteriophages. The invention also relates to methods to engineer these lactic acid bacteria as well as their use to ferment milk.

Lactic acid bacterium with a reduction of sensitivity to cos-type bacteriophages

Methods and compositions are provided for the use of anti-CRISPR (ACR) proteins in plants, including modulation of Cas endonuclease activity, improvement of frequency of homologous recombination, control of Cas endonuclease activity during various cell cycles, spatial and/or temporal regulation of Cas endonuclease activity in plants, usage in gene activation or repression, as well as reduction of off-target polynucleotide cleavage.

Anti-CRISPR polynucleotides and polypeptides and methods of use

The invention relates to anti-CRISPR genes and anti-CRISPR proteins, and their uses in various biotechnology applications.

Anti-CRISPR genes and proteins and methods of use

The invention relates to CRISPR sequences found in bifidobacteria and their uses.

Bifidobacteria CRISPR sequences

The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof.

Method of modulating cell resistance

The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.

Cultures with improved phage resistance

The present invention relates to a nucleic acid comprising aCRISPR repeat region and/or aCRISPR spacer region.

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Method for modulating resistance

The present invention provides methods for tagging and/or identifying microorganisms. In some preferred embodiments, the microorganisms are bacteria. In some particularly preferred embodiments, the bacteria are members of the genus, while in other embodiments, the bacteria are members of other genera. The present invention also provides microorganisms tagged using the methods set forth herein. In some preferred embodiments, the tagged microorganisms are bacteria. In some particularly preferred embodiments, the tagged bacteria are members of the genus, while in other embodiments, the tagged bacteria are members of other genera.

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Oregon, WI, United States of America

Dennis A Romero