Micronics, Inc.

Perkinelmer Health Sciences, Inc.

Denise Maxine Hoekstra

C Frederick Battrell

John Clemmens

John R Williford

John Gerdes

Joan Haab

Stephen Mordue

Mark Stamatios Kokoris

Isaac Sprague

Melud Nabavi

Wayne L Breidford

Elizabeth Reeves Leber

Jason Capodanno

Sabrina N Gunderson

Sabrina N Gates

Elizabeth R Leber

Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices

Disclosed herein is a device comprising a pair of bellows pumps configured for efficient mixing at a microfluidic scale. By moving a fluid sample and particles in suspension through an aperture between the paired bellows pump mixing chambers, molecular collisions leading to binding between the particles and ligands in the sample are enhanced. Such devices provide an alternative for mixing that does not use a vent and can be used with a variety of particles in suspension such as magnetic beads to capture or purify useful cells and molecules.

Microfluidic mixing and analytical apparatus

Formulations for dry storage of PCR reagents are described. These formulations find use in manufacture of self-contained microfluidic card devices for PCR clinical testing in which the reagents are reconstituted at the point of testing. In these cards, TAQ polymerase is stored 'on-board' in vitrified dry form without lyophilization or freezing, and is reconstituted by either the sample or a sample eluate during the assay.

Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.

Integrated nucleic acid assays

Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.

Methods and devices for microfluidic point-of-care immunoassays

Microfluidic mixing and analytic apparatus

Growing community of inventors

Monroe, WA, United States of America

Denise Maxine Hoekstra