Pacific Biosciences of California, Inc.

Ciena Corporation

Oni Systems Corp.

Mathieu Foquet

Mark Maxham

Paul Lundquist

Denis Zaccarin

Yves Lacroix

Stephen Turner

Paul M Lundquist

Stephen Wilson Turner

John Dixon

Hon Wah Chin

Pezhman Monadgemi

Cheng Frank Zhong

Ravi Saxena

Marc Levesque

Annette Claire Grot

Chris W Barnard

Aaron J Rulison

Holden Jessup

Rohit Sharma

Peiqian Zhao

Brian Pheiffer

Michael Eugene Durling

Yanqiao Huang

Todd Beine

Paul Mitalas

Carl Paquet

Frank Zhong

Saurin Shah

Mike Sieben

Melissa Li

Cheng-Frank Zhong

Processes for making high multiplex arrays for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. The high multiplex arrays include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

Process for making high multiplex arrays

Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

High multiplex arrays and systems

Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.

Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources

Ultra-high multiplex analytical systems and methods

Methods and systems for monitoring a plurality of different optical signals from a single source of such signals, where each such different optical signal is spatially separated from other such signals and directed to different detectors or locations upon a single detector, which direction is generally accomplished through the use of a small number of optical components and/or manipulations.

Methods and systems for monitoring multiple optical signals from a single source

An apparatus for suppressing optical power transients includes a variable optical attenuator receiving an input optical signal and outputting an output optical signal; an optical power sensing element coupled to the input optical signal and sensing a portion of the input optical signal; and a feedforward loop controller coupled to the variable optical attenuator and to the optical power sensing element; the feedforward control loop providing feedforward control of the variable optical attenuator to reduce optical power transients of the input optical signal and maintain a substantially constant output power based on the input optical power and a reference value; the variable optical attenuator having a default opaque state in which the input optical signal is substantially attenuated when power is not being supplied to said variable optical attenuator. Variations include feedback loop controllers and a combination feedback and feedforward loop controllers.

Transient optical power suppressing apparatus, method, and network

Mitigative and remedial approaches to reduction of autofluorescence background noise are applied in analytical systems that rely upon sensitive measurement of fluorescent signals from arrays of fluorescent signal sources. Such systems are for particular use in fluorescence based sequencing by incorporation systems that rely upon small numbers or individual fluorescent molecules in detecting incorporation of nucleotides in primer extension reactions.

Methods and systems for analyzing fluorescent materials with reduced authofluorescence

Growing community of inventors

San Jose, CA, United States of America

Denis Zaccarin