Aventis Pharma S.a.

Rhone-poulenc Rorer S.a.

Other

Rhodia Chimie

Sanofi-aventis

Rhone-poulenc Fibres Et Polymeres S.a.

Evologic S.a., Marliere Technologies Societe Civile

Rhone-poulenc Biochimie, Et Al.

Scientist of Fortune, S.a.

Evologic Sa

Denis Thibaut

Francis Blanche

Laurent Debussche

Joel Crouzet

Veronique Blanc

Patricia Lacroix

Nathalie Jacques

Monique Zagorec

Valerie De Crecy-Lagard

Jean-Claude Barriere

Jean-Marc Paris

Nathalie Bamas-Jacques

Beatrice Cameron

Philippe Marliere

Alain Famechon

Gilles Dutruc-Rosset

Jo&euml;l Crouzet

V&eacute;ronique Blanc

Dominique Petre

Olivier Favre-Bulle

Patrick Lefevre

Catherine Jourdat

Elisabeth Remy

Bertrand Bonnavaud

Pascal Anger

Jerome Pierrard

Didier Faucher

Volker Dóring

Carole Guitton

Sophie Levy Schil

Annett Kreimeyer

Volker Döring

Véronique Blanc

This invention relates to a process for preparing 2'-deoxynucleoside compounds or 2&#x2032;-deoxynucleoside precursors using 2-dehydro-3-deoxy-D-gluconic acid (usually abbreviated as KDG) or its salts as a starting material. A variety of 2&#x2032;-deoxynucleosides and their analogues are used as a starting material for synthesis or drug formulation in production of an antiviral, anticancer or antisense agent.

Production of 2'-deoxynucleosides and 2&#x2032;-deoxynucleoside precursors from 2-dehydro-3-deoxy-D-gluconate

The present invention relates to novel variants of the PapM polypeptide of bacteria of thegenus which possess a substrate selectivity and/or an improved effectiveness compared to the wild-type polypeptide. It also relates to the nucleic acids encoding these variants, to the microorganisms which incorporate these nucleic acids and to their use for producing B components of Streptogramins.

[object Object]

The invention concerns a method for producing a cyclic L-amino acid of formula (I), characterised in that it consists in reacting a L-diamino acid of formula (II) or an enantiomeric mixture comprising such a L-diamino acid and a corresponding D-diamino acid in variable proportions, in the presence of an ornithine cyclodeaminase or a polypeptide homologous to the ornithine cyclodeaminase. 

Stereoselective preparation of cyclic L-amino acids

The invention concerns methods for preparing pristinamycin IIA or IIB.

Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use

Novel group B streptogramin-like compounds of general formula (I), and a method for preparing streptogramines by mutasynthesis using a mutated micro-organism to influence the biosynthesis of at least one of the precursors of group B streptogramins are disclosed. Novel nucleotide sequences involved in the biosynthesis of said precursors, and their uses, are also disclosed.

Streptogramins and method for preparing same by mutasynthesis

The invention concerns methods for preparing pristinamycin IIB and methods for preparing modified pristinamycin IIB.

Novel polypeptides involved in the biosynthesis of cobalamines and/or cobamides, in particular coenzyme B , genetic material responsible for expressing these polypeptides, and a method for preparing them, are described. A method for amplifying the production of cobalamines, and particularly coenzyme B , using recombinant DNA techniques, are also described.

Methods of increasing the production of cobalamins using cob gene expression

Purified form of streptogramines, characterised in that it contains at least one component from group B streptogramines of general formula (I), wherein R is Me or Et, R is H or OH, and R is substituted benzyl of general formula (III), such that 1) if R is H, R is NR R , wherein one of R and R is H or an Me radical and the other is Me, and R&prime; is Cl or Br, or R is (C3-5)alkenyl if R and R are Me, or 2) R is H and R&prime; is halogen, alkylamino or dialkylamino, an ether-oxide residue, alkylthio, (C1-3)alkyl or trihalogenomethyl, or R is halogen, (C2-4)alkylamino, (C2-4)dialkylamino or methyl ethyl amino, pyrrolidino alkenyl alkyl amino, dialkylamino, an alkyl cycloalkylmethyl amino radical or in ether-oxide residue, alkylthio, alkylthiomethyl, (C1-6)alkyl, aryl or trihalogenomethyl, and R&prime; is H, or R is halogen, amino, alkylamino or dialkylamino, an ether-oxide residue, an alkylthio radical, (C1-6)alkyl or trihalogenomethyl and R&prime; is halogen, alkylamino or dialkylamino, an ether-oxide residue or alkylthio, (C1-3)alkyl, cocrystallised with one or more minor components of group A of general formula (II) wherein R&Prime; is H, Me or Et.

Purified form of streptogramines, preparation of same and pharmaceutical compositions containing them

The invention provides a method for preparing streptogramins using genetically-modified microorganisms to influence the biosynthesis of at least one of the precursors of the group B streptogramins. Cultures of the genetically-modified microorganisms are supplemented with a least one precursor that is different from the streptogramin precursor whose biosynthesis is altered and the streptogramins produced are recovered.

Streptogramins for preparing same by mutasynthesis

The present invention relates to a process for the enzymatic hydrolysis of polyamides 6.6 to give adipic acid monomers and hexamethylenediamine monomers. The present invention further relates to an enzyme with amidase activity particularly towards substrates of the oligomer type derived from PA 6.6 and/or PA 6, said enzyme being characterized in that it consists of a peptide sequence corresponding to SEQ ID NO: 1 in the attached sequence listing and/or at least one polypeptide homologous to this sequence. The invention further relates to the DNA coding for said enzyme and to the biological precursors thereof The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.

Enzymes and micro organisms with amidase activity which hydrolyze polyamides

The invention concerns mutant microorganisms blocked in a step of Streptogramin biosynthesis and methods for preparing cells blocked in a step of Streptogramin biosynthesis.

Biosynthesis method enabling the preparation of cobalamins

Disclosed is the nucleic acid sequences for polypeptides and enzymes involved in the biosynthesis of streptogramins from Streptomyces sp. and the corresponding amino acid sequences. Also disclosed are recombinant vectors and cells as well as methods of making the polypeptides, enzymes and streptogramins using the recombinant host cells.

Polypeptides involved in the biosynthesis of streptogramins, nucleotide

This invention concerns a process for enzymatically preparing a compound of formula (1) ##STR1## from the compound of formula (2) ##STR2## by way of a microorganism or an acellular preparation derived from this microorganism. The microorganism and acellular preparation oxidizes the 2-3 bond of the macrolactones' D-proline into dehydroproline.

Process for the enzymatic preparation of macrolactone

Growing community of inventors

Paris, France

Denis Thibaut