Abbott Laboratories Corporation

Denis R Henrard

Christopher Bieniarz

Jeffrey B Huff

Intercalator compounds of formula I-T.sub.m as defined herein are provided which are comprised of intercalator moieties, or substituted intercalator moieties, having one or more functionalized chains, or moieties, and which compounds provide a high affinity for binding to the DNA molecule and show reduced self-quenching, while providing superior transport kinetics. The compounds have been found to provide enhanced fluorescence when bound to a DNA molecule within a fluorescent flow cytometry environment which is about eight to ten times brighter in fluorescence than 'bis' structure conventional intercalating agents and other known intercalating agents utilized in flow cytometry environment.

Intercalators having affinity for DNA and methods of use

Intercalator compounds are provided which are comprised of intercalator moieties, or substituted intercalator moieties, having one or more functionalized chains, or moieties, and which compounds provide a high affinity for binding to the DNA molecule and show reduced self-quenching, while providing superior transport kinetics. The compounds have been found to provide enhanced fluorescence when bound to a DNA molecule within a fluorescent flow cytometry environment which is about eight to ten times brighter in fluorescence than 'bis' structure conventional intercalating agents and other known intercalating agents utilized in flow cytometry environment.

Phenanthridium compounds are provided which are comprised of intercalators and substituted intercalator segments having a functionalized chain or chains which provide a high affinity for binding to the DNA molecule and show reduced self-quenching, while providing superior transport kinetics. The compounds have been found to provide enhanced fluorescence when bound to a DNA molecule within a fluorescent flow cytometry environment which is about eight to ten times brighter in fluorescence than 'bis' structure intercalators and other known intercalators utilized in flow cytometry environment, said enhancement of fluorescence allows a drastic reduction in the amount of intercalator needed for applications of similar sensitivity than those using known intercalators. Substantial improvements in material use efficiency, accuracy and dependability of various DNA studies are direct results of the inventive intercalator compounds, said compounds having use in known DNA intercalator applications inclusive of flow cytometry, gel electrophoresis, DNA detection, immunoassay for DNA, fluorescence in situ hybridization assays, DNA sequencing application, therapeutic application and conversion staining methodologies.

Growing community of inventors

Lake Forest, IL, United States of America

Denis R Henrard