Ultima Genomics, Inc.

Bio-Rad Laboratories, Inc.

10X Genomics, Inc.

Denis Pristinski

Gilad Almogy

Nathan Beckett

Steven Menchen

Kristopher Barbee

Mark Pratt

Avishai Bartov

Jamie Michael Sullivan

Dumitru Brinza

Abizar Lakdawalla

Derek Schulte

David P Stumbo

Jacob A Wolf

Nathan Caswell

George Carman

Gene Polovy

Augusto Manuel Tentori

Dmitri Simonian

Phillip You Fai Lee

Joshua T Oen

John Dzenitis

Joseph Anthony

Jose Martin Sosa

David Glade

Stephanie Kubecka

Steve Hobbs

Osip Schwartz

Rajiv Bharadwaj

Anthony Makarewicz

Hanyoup Kim

Bill Kengli Lin

Neil Ira Weisenfeld

Ashkan Beyranvand Nejad

Preyas Shah

Pratomo Alimsijah

David Michael Patterson

Anthony Joseph Makarewicz, Jr

Siyuan Xing

Dongyao Li

Dieter Wilk

Yunfeng Ling

William Nitsch

Reynaldo Farias Zorrilla

Steven William Short

Phillip You Lee

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.

Methods and systems for analyte detection and analysis

Provided herein are materials and methods for in situ labeling of a biological sample for use in spatial analysis of one or more analytes in the biological sample. In some aspects, in situ labeling of a biological sample includes labeling the biological sample with one or more spatial barcodes.

In situ spatial barcoding

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

System and method for droplet detection

A method of self-testing an imaging system of a sample handling apparatus is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

Self-test for imaging device

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

Methods, devices, and systems for analyte detection and analysis

System, including methods and apparatus, for optical detection. The system may comprise a light source to generate a beam of light, an optical element including a light guide and an exit window, and at least one detector.

Optical detection system with light sampling

Detection system comprising an examination region, a one-piece optical element including a focusing portion to concentrate light received from the examination region and a guiding portion to homogenize light received from the focusing portion, and a detector configured to detect homogenized light received from the guiding portion.

Detection system with one-piece optical element to concentrate and homogenize light

Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.

Methods for biological sample processing and analysis

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Dublin, CA, United States of America

Denis Pristinski