Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Commissariat a L'energie Atomique

Denis Menut

Nicolas Ugolin

Sylvie Chevillard

Julien Le Meur

Nadine Coulon

Pascal Wodling

Emilie Bosc

Jean-Marc Joseph Desaulniers

The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following, steps: a) at least two laser beams (F&#x2033;) are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and analyzed for each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the qualified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations of the latter in mixtures of the quantified element and exogenous element in known proportions.

Method and device for high speed quantitative measurement of biomolecular targets on or in biological analysis medium

The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip () of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points () each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam () is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be quantified and given concentrations of this element.

Method for the quantitative measurement of biomolecular targets deposited on a biochip, and device for implementing it

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Creteil, France

Denis Menut