Shanhai Hua Yi Bio-Tech Lab

Shanghai Huayi Bio-Lab Co., Ltd.

Shanghai Huayi Biotech Lab

Shanghai Benemae Pharmaceutical Corporation

Shanghai Huayi Bio-Lab., Ltd.

Dengxi Wu

Zhiyong Zhu

Yukun Sun

Wen Qing Chen

Gang Yu

Aizhen Wu

Jiaxiang Zhou

Shaoling Zhao

Chunjuan Shen

The present invention discloses a method of producing polypeptides, including insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs, by ligating genes in a tandem way. Also disclosed are the recombinant polypeptides produced by such a method. Using the method of this invention, 1 to 32 copies of GLP-1 (7-36) and/or GLP-1 analog genes may be expressed in tandem and the desired polypeptide can be obtained after cleavage of a fusion protein and further processes of separation and purification thus making possible the production of recombinant polypeptides, including recombinant GLP-1 (7-36) and/or GLP-1 analogs on a large scale, at a significantly reduced production cost.

Method of producing insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs

The present invention is directed to the development of novel exendin-4 derivatives exhibiting advantageous glucose-regulatory properties, and to methods of producing these derivatives, including recombinant methods in which these derivatives are produced by cleavage of a fusion protein containing multiple copies of the exendin-4 derivative peptide. The methods of the present invention can be used to simplify the process of producing the disclosed exendin-4 derivatives, thereby lowering the cost of their production.

Derivatives of the insulinotropic peptide exendin-4 and methods of production thereof

The present invention provides a Magainin derivative peptide and method of production thereof. Also provided is a pharmaceutically composition comprising said Magainin derivative peptide and pharmaceutically acceptable carrier and/or pharmaceutically compatible binding agents. The Magainin derivative peptide of the present invention having amino acid sequence of the general formula shown as below:  in which: X is an amino acid residue selected from the group consisting of Met, Ile and Leu; Y is an amino acid residue selected from the group consisting of Ser, Lys, Ile, Arg and Leu; and Z is Arg.

Derivatives of magainin and methods of production thereof

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Shanghai, China

Dengxi Wu