University of Virginia Patent Foundation

Delphine Le Roux

James P Landers

Daniel G Mills

Brian Root

An-Chi Tsuei

Christopher Birch

Jingyi Li

Jacquelyn A DuVall

Daniel A Nelson

Brandon L Thompson

A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.

Devices and methods for extraction, separation and thermocycling

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Barboursville, VA, United States of America

Delphine Le Roux