Zhejiang Hisun Pharmaceutical Co., Ltd.

Dejin Fu

Liang Zhang

Zhiqing Yang

Xiangyang Zhang

Ling Zhang

Hongying Luo

Hairong Luo

Yong Yang

Hua Chen

Zhenjuan Shi

Wei Yan

Weijiang Wen

Di Su

Xiande Gu

Xiaojun Ma

Kaijun Wang

A method for enzymatic preparation of fludarabine phosphate, comprising reaction of fludarabine with a high-energy phosphate compound under the action of deoxyribonucleic acid kinase. According to said method, acetate kinase and acetyl phosphate free acid or acetyl phosphate are also added. The technical problems present in the existing processes are successfully addressed by employing the enzymatic process to prepare the fludarabine phosphate. The usage of the high-energy phosphate compound is reduced by means of adding acetate kinase to recycle and regenerate a small amount of the high-energy phosphate compound, thereby reducing the generation of by-products having similar structures to the fludarabine phosphate, enhancing the operation convenience of purification steps in the industrial production of the fludarabine phosphate. The process is environment friendly, the reaction conditions are moderate, the cost is low, and the yield and the purity of the product obtained are high.

Method for enzymatic preparation of fludarabine phosphate

Provided are a new crystal form of fludarabine phosphate, a preparation method therefor, a pharmaceutical composition containing same, and an application thereof in preparing medicine. Crystal forms I and II of the fludarabine phosphate have excellent properties in terms of solubleness, dissolution rate, chemical stability, and processing adaptability.

Crystal form of fludarabine phosphate, preparation method therefor, and application thereof

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Zhejiang, China

Dejin Fu