Indian Oil Corporation Limited

Department of Biotechnology

Other

Lanzatech New Zealand Limited

Dbt-ioc Centre for Advanced Bio-energy Research

Indian Oil Corporaton Limited

Deepak Kumar Tuli

Akhilesh Kumar Bhatnagar

Rakesh Sarin

Ravi Prakash Gupta

Madan Mohan Rai

Anshu Shankar Mathur

Sobhan Ghosh

Krishan Kumar Swami

Sabyasachi Sinha Ray

Suresh Kumar Puri

Ram Prakash Verma

Simranjeet Kaur Sandhu

Mukund Adsul

Reeta Rani Singhania

Sankara Sri Venkata Ramakumar

Niranjan Raghunath Raje

Shanti Parkash

Khem Chand Dohhen

Swaminathan Sivaram

Ajay Kumar Arora

Pankaj Kumar Mondal

Manoj N Kumar

Ravinder Kumar Malhotra

Sean Dennis Simpson

Alex Cheru Pulikottil

Mahendra Pratap Singh

Ravindra Kumar

Alok Satlewal

Sebastian Michal Bernasek

Rajiv Ranjan

Rajeev Ranjan

Thekke Pangil Mohandas

Mallinamadugu Jogimarappa Yanjarappa

Dheer Singh

Biswajit Basu

Manoj Kumar

Anand Kumar

Ruchi Agrawal

Ajay Kumar Sharma

Dattatraya Haribhau Gholap

Ashok Kumar Tiwari

Preeti Mehta

Shanti Prakash

Ajay Kumar

Tirath Raj

Manas Ranjan Swain

Ajit Singh

Jitendra Kumar Saini

Brij Mohan Bansal

Ruchi Gaur

Pankaj Kumar Mandal

Manali Kapoor Kakkar

Anshu Shanker Mathur

Anil Kumar Patel

Dilip Singh

Meeta Sharma

Prabhat Kumar Saxena

Harish Chander Bhatia

Mahendra Pratap Indian Oil Corporation Limited Singh

Wadhava Ram Kalsi

The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture ofMRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.

Process for simultaneous production of citric acid and cellulolytic enzymes

The present invention relates to a mutant fungal strain of'MRJ-16' characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, &#x3b2;-glucosidase, endoglucanase, &#x3b1;-L arabinofuranosidase, &#x3b2;-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naivestrain NCIM 1228, when used in a fermentation process. The mutant strain 'MRJ-16' with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

Method for preparation and screening of fungal mutant with high hydrolytic activity and catabolite derepressed character

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and &#x3b2;-glucosidase were 1.5 times higher thanMRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

Method for preparation of fungal mutant with high hydrolytic activity

The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and &#x3b2;-glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water usingMRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

Bio-refinery waste utilization for enzyme production using novel penicillium funiculosum MRJ-16 fungal strain

The present invention relates to a method of cellulases enzymes production byMRJ-16 using minimum media components and/or low cost media components like cellulose or pretreated lignocellulosic biomass as carbon source and soya flour or defatted soya flour or de-oiled soya cake as nitrogen source. The present invention also provides a method for production of high titer of cellulases and hemicellulases enzymes usingMRJ-16 using minimum media and/or low cost media components.

Provident method of cellulases enzymes production byMRJ-16 using low cost media components

The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO, COor Hor mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.

Fermentation process for the production of lipids

The present invention relates to an additive or additive composition for improved production of fermentable sugars from lignocellulosic biomass during enzymatic hydrolysis. More particularly, the present invention discloses petrochemical waste as an additive or an additive composition containing one or more ethylene glycol oligomers and one or more metal ions which are useful for improved production of fermentable sugars from lignocellulosic biomass during enzymatic hydrolysis.

Method for improved production of fermentable sugars from lignocellulosic biomass using additive and enzyme

The present invention discloses a method for producing readily available and hydrolysable polysaccharide-enriched biomass from lignocellulosic biomass while quantitatively retaining carbohydrate and producing fewer amounts of inhibitors. The method includes treating lignocellulosic biomass with an aqueous solution at elevated temperatures in a sequential step using hydrazine hydrate and sodium sulphite. In accordance with the present invention, the biomass may be further treated with a saccharification enzyme consortium to produce fermentable sugars.

Method of pretreatment for enhanced enzymatic hydrolysis

The present invention relates to a process for enhancing hydrogen production in an anaerobic fermentative hydrogen production process. The present invention also provides a production media, more specifically, a sugar production media for use in an anaerobic fermentative hydrogen production process to enhance hydrogen production. The present invention also provides a novel strainsp., MTCC 25082 for use in an anaerobic fermentative hydrogen production process for enhancing hydrogen production.

Formulation and process for biohydrogen production

The present invention relates to the continuous process of sequestration of nutrients from waste effluents released from gas fermentation plants or green-house gases emission managing plants by novel Thraustochytrids. Particularly this invention relates to the methods and systems to enhance sequestration rate and productivity of the process. This invention also relates to rapid biotransformation of nutrients present in waste effluents into high value omega-3 fatty acids like Docosahexaenoic acid (DHA), Docosapentaenoic acid (DPA), Eicosapentaenoic acid (EPA) and lipids for biodiesel. This disclosure is about means of processing of waste streams and producing value added products out of it.

Thraustochytrid based process for treating waste effluents

The present invention provides a cost effective biotechnological process for production of bio-fuels from isolated and characterized microalgae. The algal strains used in the present invention having higher biomass, higher lipid productivity, higher pH and temperature tolerance are selected from the group consisting ofiOC-1,iOC-2,andor a combination thereof having 95-100% similarity with 18s ribosomal nucleic acids nucleotide sequences (rDNA) given for Seq. ID I, Seq. ID 2, Seq. ID 3, Seq. ID 4, Seq. ID 5 and Seq. ID 6. The present process of bio-fuel production comprises the steps of producing lipid from green algae in bioreactors by various novel steps and extracting oil from dried algal cells and ultimately producing biodiesel by transesterification of the said extracted oil.

Process for producing lipids suitable for biofuels

The present invention provides a bio-augmentation composition for improving the hydrocarbon degradation efficiency of effluent treatment plant for hydrocarbon degradation in wastewater generated from hydrocarbon processing industry and a method thereof. The composition comprises a synergistic combination of selective microorganisms to develop a consortium enabling effective degradation of hydrocarbons present in wastewater and converting thereof into harmless and environment friendly substances. The invention also provides for the said microorganisms and their isolations.

Bio-augmentation composition and use thereof for improving efficiency of effluent treatment in hydrocarbon processing plant

The present invention relates to a additive composition for use as lubricity improver for low sulphur diesel, comprising c) 0.1-10% by weight of ester derivative derived from cashew nut shell liquid (CNSL esters) of formula (I); f) 0.1-10% by weight of ester derivative derived from cashew nut shell liquid of formula (II); g) 50-95% by weight of free fatty acid of the formula RCOOH in which R represents an alkyl/alkenyl group with 12 to 24 carbon atoms. h) 1-30% by weight of synthetic esters derived by esterifying tri, tetra, penta hydric alcohols with carboxylic acids such as lauric, palmitic, linoleic, ricinoleic etc. 

Lubricity improving additive composition for low sulfur diesel fuel

The present invention provides a fuel additive composition for stabilizing blends of ethanol and a hydrocarbon boiling in the gasoline or diesel range, comprising: a) 0.1-10% of Cashew Nut Shell Liquid (CNSL) derivative(s) or mixtures thereof of formula (I) where m=0-12, n=0, 2, 4 & 6 and b) 0.1-10% of an organic co-solvent depending upon the percentage composition of diesel and ethanol blend. 

Fuel additive composition for stabilising blends of ethanol and a hydrocarbon

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Faridabad, India

Deepak Kumar Tuli