Htg Molecular Diagnostics, Inc.

Debrah Thompson

Bruce Seligmann

Tom Vasicek

Matthew Rounseville

Debra A Gordon

The present disclosure provides methods and kits for direct sequencing of nucleic acid targets. Such methods can be used to determine if one or more nucleic acid targets are present in a sample.

Method of direct target sequencing using nuclease protection

The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5'-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.

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Tucson, AZ, United States of America

Debrah Thompson