Affymetrix, Inc.

Affymax Technologies, N.v.

Debra A Ross

James L Winkler

Lois Aldwin

Christopher J Buchko

Douglas N Modlin

Stephen P A Fodor

Stephen P Fodor

A method and device for forming large arrays of polymers on a substrate (). According to a preferred aspect of the invention, the substrate is contacted by a channel block () having channels () therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodologies.

Combinatorial strategies for polymer synthesis

A method and device for forming large arrays of polymers on a substrate (). According to a preferred aspect of the invention, the substrate is contacted by a channel block () having channels () therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

A method for producing arrays by spacing a dispenser a distance from a surface of a support, dispensing a volume containing a compound in a single coupling step of less than 5 nl to occupy a localized area of less than 1 cmof the surface of the support, allowing the compound to bind directly or indirectly to the support and repeating the steps to produce an array of at least 100 ligands at a density of 1000 per cmor greater.

A method for producing polymer arrays by spacing a dispenser a distance from a surface of a support, dispensing a volume containing a monomer in a single coupling step of less than 5 nl to occupy a localized area of less than 1 cmof the surface of the support, allowing the monomer to bind directly or indirectly to the support and repeating the steps to produce an array of at least 100 polymer ligands at a density of 1000 per cmor greater.

A method for producing arrays by depositing a resist on a substrate, selectively removing a portion of the resist to expose localized areas, dispensing a monomer to occupy a localized area of less than 1 cmof the surface of the support, allowing the monomer to bind directly or indirectly to the support and repeating the steps to produce an array of at least 10 different polymers is formed.

A device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Combinatorial kit for polymer synthesis

An array is created by moving a dispenser toward a solid support until a tip of the dispenser touches the support, withdrawing the tip from the surface and releasing a drop of 5 nanoliters or less in an area smaller than 1 mm.sup.2 to create an array of at least 100 spots.

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are flowed through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Very large scale immobilized polymer synthesis using mechanically

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

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Fremont, CA, United States of America

Debra A Ross