Novartis Vaccines & Diagnostics, Gmbh

Chiron Corporation

Ciba Corning Diagnostics Corp.

Chiron Diagnostics Corporation

Deborah Lynn Parkes

Paul A Luciw

Dino Dina

Philip J Barr

Carlos George-Nascimento

Martha Truett

Kathelyn S Steimer

Ray Sanchez Pescador

Rob Hallewell

Stephen Ralph Coates

Gary Van Nest

Gary A Van Nest

Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.

Purified pol DNA, a recombinant DNA construct for expression of HIV pol polypeptide sequences and a cell containing such construct

Method for expressing HIV polypeptides

Recombinant DNA construct for HIV envelope polypeptides and polypeptides produced thereby

Human immunodeficiency virus (HIV) nucleotide sequences

HIV immunoassays using gag polypeptides

Disclosed are recombinant, synthetically and otherwise biologically produced novel proteins and polypeptides which are encoded by the DNA sequence for HSV-2 glycoprotein G (gG) or fragments of said gG sequence particularly by the unique sequence for gG or portions of said unique sequence. The unique sequence proteins and polypeptides are serologically active, can be produced easily and safely at low cost, are useful as diagnostic reagents for HSV-2 and as vaccines against HSV-2. Further disclosed are serological assays based on such unique sequence gG proteins and polypeptides that diagnose the presence of herpes simplex virus type 2 (HSV-2) specific antibodies and can differentiate between HSV-2 and herpes simplex virus type 1 (HSV-1) specific antibodies. Such assays are useful to diagnose genital infections, to detect for exposure to HSV-2 and to screen pregnant women to protect newborns from neonatal HSV-2 infection. Also disclosed are antibodies to such gG proteins and polypeptides which are useful therapeutically diagnostically and for affinity purification. Further, disclosed are purified and isolated DNA molecules which can be used as probes specific for HSV-2 DNA.

Herpes simplex virus type 2-glycoprotein G proteins and polypeptides

Disclosed are recombinant, synthetically and otherwise biologically produced novel proteins and polypeptides which are encoded by the DNA sequence for HSV-2 glycoprotein G (gG) or fragments of said gG sequence, particularly by the unique sequence for gG or portions of said unique sequence. The unique sequence proteins and polypeptides are serologically active, can be produced easily and safely at low cost, are useful as diagnostic reagents for HSV-2 and as vaccines against HSV-2. Further disclosed are serological assays based on such unique sequence gG proteins and polypeptides that diagnose the presence of herpes simplex virus type 2 (HSV-2) specific antibodies and can differentiate between HSV-2 and herpes simplex virus type 1 (HSV-1) specific antibodies. Such assays are useful to diagnose genital infections, to detect for exposure to HSV-2 and to screen pregnant women to protect newborns from neonatal HSV-2 infection. Also disclosed are antibodies to such gG proteins and polypeptides which are useful therapeutically, diagnostically and for affinity purification. Further, disclosed are purified and isolated DNA molecules which can be used as probes specific for HSV-2 DNA.

DNA sequence for the unique sequence herpes simplex virus type

Novel methods and compositions are provided for vaccinating a host susceptible to viral infection. Particularly, an initial injection is employed of one or more polypeptides associated with the host immune response to the pathogen followed by administration of the pathogen in attenuated form, particularly having a deletion associated with the gene expressing at least one of said polypeptides. In this manner, hosts may be vaccinated so as to maintain a strong defensive posture against infection from the pathogen. The method is specifically illustrated with feline leukemia virus.

Compositions and methods for FeLV vaccination

Methods and compositions are provided for the efficient production of a polypeptide having immunological reactivity corresponding to that of naturally-occurring envelope glycoproteins of feline leukemia virus. A DNA construct including a replication system recognized by yeast, and a viral envelope protein gene under the transcriptional control of a yeast promoter, and terminator is provided. By transforming a yeast host with the DNA construct, enhanced yields of the product may be obtained. Saccharomyces carlsbergensis strains 2150-2-3 (pCP-envB-R) and 2150-2-3 (pCP-envA-R) were deposited at the American Type Culture Collection on July 18, 1984, and granted accession nos. 20720 and 20721, respectively.

Feline leukemia virus protein vaccines

Growing community of inventors

Oakland, CA, United States of America

Deborah Lynn Parkes