Powderject Vaccines, Inc.

Other

Powderject Research Limited

Deborah L Fuller

James T Fuller

Joel R Haynes

Georg Widera

Timothy Shipley

Mary Wu

David Francis Sarphie

William F Swain

Ning-Sun Yang

Lee K Roberts

Joseph K Burkholder

Michael D Macklin

Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

Advanced genetic vaccines

Adjuvanted genetic vaccines

Recombinant nucleic acid molecules are described. The molecules have a sequence or sequences encoding at least twoantigens. Vectors and compositions containing these molecules are also described. In addition, compositions containing a cocktail of recombinant nucleic acid molecules having a sequence or sequences encoding one or moreantigens are described. Methods of eliciting an immune response using these molecules and compositions are also described.

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Recombinant nucleic acid molecules are described. The molecules have a first sequence encoding a Hepatitis B virus core antigen and a second sequence encoding at least one T cell epitope inserted within the first sequence. Vectors and compositions containing these molecules are also described. Methods of eliciting an immune response using these molecules are also described.

Nucleic acid constructs

Methods for assessing immunocompetence, cellular or humoral immunity, antigen exposure, or allergic conditions in an individual by accelerating diagnostic particles into a target skin site in the individual are provided.

Immunodiagnostics using particle delivery methods

A method of genetically transforming mammalian unattached cells is disclosed. The method begins by preparing copies of a nucleic acid construct and coating these copies onto biologically inert carrier particles. Mammalian unattached cells are isolated in a liquid suspension. The cell suspension is placed on a target surface, wherein the liquid is spread to a thin film on the target surface. In an alternative embodiment of the present invention, the liquid is spread onto a porous surface. The cells are bombarded with the construct-coated particles in such a fashion that some particles lodge in the interior of at least some of the cells. The existence and expression of the construct within the cell is verified.

Particle-mediated transformation of mammalian unattached cells

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Middleton, WI, United States of America

Deborah L Fuller