The Broad Institute, Inc.

The General Hospital Corporation

The Brigham and Women's Hospital, Inc.

Massachusetts General Hospital Corporation

Massachusetts Institute of Technology

Harvard College

The Board Institute, Inc.

Deborah Hung

Tomohiko Kawate

Sarah Stanley

Roby Bhattacharyya

Motohisa Shimizu

Noriakie Iwase

Sarah Grant

Jonathan Livny

Amy Barczak

James Gomez

Feng Zhang

James Joseph Collins

Benito Munoz

Omar O Abudayyeh

Jonathan S Gootenberg

Michael H Serrano-Wu

Eric Lander

Paul Blainey

Timothy A Lewis

Sivaraman Dandapani

Partha Pratim Nag

Pardis Sabeti

Mark Borowsky

Cameron Myhrvold

Milesh Patel

Noriaki Iwase

Peijun Ma

Gowtham Thakku

Cheri Ackerman

Lisa Cosimi

The present disclosure relates to compositions, methods, and kits for rapid phenotypic detection of antibiotic resistance/susceptibility.

Compositions and methods for detecting antibiotic responsive mRNA expression signatures and uses thereof

The present disclosure relates to method of distinguishing between two or more species of one or more organisms in a sample, by contacting a biological sample comprising ribosomal ribonucleic acid (rRNA) with a set of antisense probes, wherein the set of probes contains at least one detectable probe that is specific for a target rRNA sequence of each species to be tested, and wherein the individual probes specific for each species comprises less than about 85% sequence identity; and, detecting hybridization between one or more of the probes and the rRNA, thereby distinguishing between two or more species in a sample.

Ribosomal ribonucleic acid hybridization for organism identification

The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

CRISPR effector system based diagnostics

The specification relates generally to methods of detecting, diagnosing, and/or identifying pathogens, e.g., infectious disease pathogens and determining their drug sensitivity and appropriate methods of treatment. This invention also relates generally to methods of monitoring pathogen infection in individual subjects as well as larger populations of subjects.

Methods of diagnosing infectious disease pathogens and their drug sensitivity

The invention relates to compounds of Formula I or a pharmaceutically acceptable salt, ester or prodrug thereof: 

Compounds for the treatment of mycobacterial infections

The invention relates to compounds of Formula I or a pharmaceutically acceptable salt, ester or prodrug thereof:

Disclosed are compounds that can be used for treating tuberculosis.

Compounds for the treatment of tuberculosis

This disclosure relates to a method for increasing the hybridization efficiency of a probe and a target RNA in a sample, for example to identify a particular RNA present in the sample. The method includes heating a lysate sample comprising at least one target RNA, such as a tRNA, mRNA or rRNA, at a temperature of about 95&#xb0; C. for a time sufficient to interfere with secondary structure of the RNA, wherein the time is short enough, such that the RNA in the cell lysate sample are not significantly degraded, and wherein the lysate comprises a cell lysis buffer comprising a chemical denaturant. To detect a target RNA in the lysate, the lysate is contacted with at least one detectable probe, such as a labeled probe, designed to specifically hybridize to the target RNA in the lysate.

Enhanced methods of ribonucleic acid hybridization

Disclosed ore substituted aminothiazoles, which can be used for, among other things, the treatment of tuberculosis, pharmaceutical compositions containing the same, and methods of using the same.

Substituted aminothiazoles for the treatment of tuberculosis

Rapid phenotypic diagnosis of pathogens and drug resistance using transcriptional expression signatures

Growing community of inventors

Cambridge, MA, United States of America

Deborah Hung