Braskem S.a.

Debora Noma Okamoto

Iuri Estrada Gouvea

Lucas Pedersen Parizzi

Alessandro Silva Nascimento

Igor Polikarpov

Verônica Leite Queiroz

Rafael Victório Carvalho Guido

Daniel Johannes Koch

Veronica Leite Queiroz

Mateus Schreiner Lopes

Rafael Victorio Carvalho Guido

The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

NADH-dependent enzyme mutants to convert acetone into isopropanol

The present application relates to recombinant microorganisms expressing a dehydratase useful in a one-step, direct fermentative production of one or more primary alkenes from one or more saturated primary or secondary alcohols. Known, well developed high-yielding pathways that use renewable feedstock can be introduced into the recombinant microorganisms to obtain the alcohol precursors. Also provided are methods of producing one or more primary alkenes using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the primary alkene products.

Formation of alkenes through enzymatic dehydration of alkanols

Growing community of inventors

Campinas, Brazil

Debora Noma Okamoto