Rutgers, The State University of New Jersey

Sloan-kettering Institute for Cancer Research

Debabrata Banerjee

Joseph R Bertino

Michel Sadelain

Dimitris Metaxas

Tamara Minko

Xiaolei Sharon Huang

Olga B Garbuzenko

John E Kerrigan

Emine E Abali

Kathleen W Scotto

Emine A Ercikan-Abali

Shin Mineishi

Xiaoqi Xie

Youzhi Tong

Xinyue Liu-Chen

A system and methods for generating 3D images from 2D bioluminescent images and visualizing tumor locations are provided. A plurality of 2D bioluminescent images of a subject are acquired using any suitable bioluminescent imaging system. The 2D images are registered to align each image and to compensate for differences between adjacent images. After registration, corresponding features are identified between consecutive sets of 2D images. For each corresponding feature identified in each set of 2D images, an orthographic projection model is applied, such that rays are projected through each point in the feature. The intersection points of the rays are plotted in a 3D image space. All of the 2D images are processed in the same manner, such that a resulting 3D image of a tumor is generated.

System and method for generating three-dimensional images from two-dimensional bioluminescence images and visualizing tumor shapes and locations

The instant invention provides amino acid sequences competing with E2F for DNA binding. Methods of using said amino acid sequences for treatment of hormone-refractory prostate cancer are also provided.

E2F as a target of hormone refractory prostate cancer

A system and methods for generating 3D images () from 2D bioluminescent images () and visualizing tumor locations are provided. A plurality of 2D bioluminescent images of a subject are acquired during a complete revolution of an imaging system about a subject, using any suitable bioluminescent imaging system. After imaging, the 2D images are registered () according to the rotation axis to align each image and to compensate for differences between adjacent images. After registration (), corresponding features are identified between consecutive sets of 2D image (). For each corresponding feature identified in each set of 2D images an orthographic projection model () is applied, such that rays are projected through each point in the feature. The intersection point of the rays are plotted in a 3D image of a tumor is generated. The 3D image can be registered with a reference image of the subject, so that the shape and location of the tumor can be precisely visualized with respect to the subject.

System and methods for generating three-dimensional images from two-dimensional bioluminescence images and visualizing tumor shapes and locations

New mutant forms of human dihydrofolate reductase (DHFR) which have properties superior to the previously disclosed mutants have mutations at both amino acidand amino acid. Specific mutant forms are Ser31Tyr22, Ser31Phe22, Gly31Tyr22, Gly31Phe22, Ala31Tyr22 and Ala31Phe22. The mutant DHFR of the invention may be used as a selectable marker, and to modify the genome of human cells, particularly bone marrow cells or peripheral blood stem cells, to render them resistant to chemotherapy using antifolate agents.

Double mutants of dihydrofolate reductase and methods of using same

New mutant forms of human dihydrofolate reductase (DHFR) which have properties superior to the previously disclosed mutants have mutations at both amino acid 22 and amino acid 31. Specific mutant forms are Ser31Tyr22, Ser31Phe22, Gly31Tyr22, Gly31Phe22, Ala31Tyr22 and Ala31Phe22. The mutant DHFR of the invention maybe used as a selectable marker, and to modify the genome of human cells, particularly bone marrow cells or peripheral blood stem cells, to render them resistant to chemotherapy using antifolate agents.

The present invention provides a mutated human TS, said mutated synthase differing from wild type TS at amino acid residue 49, amino acid residue 52, amino acid residue 108, amino acid residue 221 or amino acid residue 225. Also provided is cDNA mutated human TSs and novel vectors and host cells and methods of using the mutated human TSs.

Growing community of inventors

Bellerose, NY, United States of America

Debabrata Banerjee