Wisconsin Alumni Research Foundation

Deane F Mosher

Daniel P Aeschlimann

Jane M Sottile

William J Checovich

Anna Huttenlocher

Shinji Asakura

Valeriu Bortnov

Frances M Smith

Ruth Anne Houseright

A method to inhibit neutrophil recruitment to damaged tissue, thereby inhibiting inflammation in a subject. The method includes administering to an anti-inflammatory amount of a myeloid-derived growth factor ('MYDGF'). Also disclosed are corresponding pharmaceutical compositions of matter containing the MYDGF.

Method to inhibit neutrophil recruitment to damaged tissue using myeloid-derived growth factor

A pair of degenerate oligonucleotide primers can amplify transglutaminase-specific fragments of known transglutaminase genes. The primers are also used to obtain new transglutaminase gene products. The nucleotide sequence of a novel transglutaminase gene (termed TG.sub.X) is presented.

Transglutaminase and gene encoding same

Lysophosphatidic acids are used to enhance fibronectin binding. This assists in the process of wound healing. The acids are exogenously supplied. In one aspect, a kit is provided containing a skin salve that contains the acid or a salt thereof.

Use of lysophosphatidic acids to enhance fibronectin binding

Recombinant protein purification vectors and methods for their use are disclosed. The vectors contain a DNA sequence coding for a gelatin binding region of fibronectin. The vectors express a foreign DNA sequence of interest fused to the fibronectin portion. Secretion signals on the fused product assist the product in being secreted from a production cell. The product can then be purified on a gelatin-containing affinity column and digested with a protease, such as trypsin, to cleave the desired protein from the gelatin binding region. The protein then contains no more than one extraneous amino acid.

Fibronectin purification vector

Recombinant protein purification vectors and methods for their use are disclosed. The vectors contain a DNA sequence coding for a gelatin binding region of fibronectin. The vectors express a foreign DNA sequence of interest fused to the fibronectin portion. Secretion signals on the fused product assist the product in being secreted from a production cell. The product can then be purified on a gelatin containing affinity column and digested with a protease such as trypsin to cleave the desired protein from the gelatin binding region. The vectors can also be designed to code for factor XIIIa cross liking sites and to have a chemically reactive cysteine residue.

Disclosed herein are methods for using a proteinaceous material present in kinin free high molecular weight kininogen to treat surfaces to prevent or minimize adhesion by blood components and/or animal cells. For example, in medical applications, one can treat plastic tubes or other conduits that carry blood to reduce the tendency of the blood to block the conduit. Also disclosed is an improved method of purifying kinin free high molecular weight kininogen.

Methods to make and use proteinaceous material present in kinin-free

A method for preparation of multimeric fibronectin. Dimeric fibronectin is treated with guanidine. The dimeric fibronectin so treated is then incubated to allow multimeric fibronectin to form therefrom.

Process for preparation of multimeric plasma fibronectin

A monoclonal antibody produced by a hybridoma formed by the fusion of cells from a mouse myeloma line and spleen cells from a mouse previously immunized with thrombospondin. The monoclonal antibody reacts with human, bovine, and canine thrombospondin but not with the thrombospondin present in rabbit serum. The monoclonal antibody is capable of being used to identify human, bovine, and canine thrombospondin in ELISAs and by means of immunohistological techniques and also can be used to isolate such thrombospondin by when employed in immunoisolation techniques.

Growing community of inventors

Madison, WI, United States of America

Deane F Mosher