Ninebot (beijing) Tech Co., Ltd

Genetron Health (beijing) Co., Ltd.

Dayong Xu

Wei Li

Lei Liu

Haoran Liu

Liying Li

Zhilin Wu

Sizhen Wang

Yuchen Jiao

Hai Yan

Qiaosong Zheng

Xiao Shi

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The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: a forward fusion primer designed according to the target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.

Method for rapidly constructing amplicon library through one-step process

Growing community of inventors

Beijing, China

Dayong Xu