Massachusetts Institute of Technology

Firefly Bioworks, Inc.

Davide M Marini

Douglas P Hart

Federico Frigerio

Daniel Colin Pregibon

Andreas Windemuth

Isaac Stoner

Timothy Erps

The attenuation and other optical properties of a medium are exploited to measure a thickness of the medium between a sensor and a target surface. Disclosed herein are various mediums, arrangements of hardware, and processing techniques that can be used to capture these thickness measurements and obtain three-dimensional images of the target surface in a variety of imaging contexts. This includes general techniques for imaging interior/concave surfaces as well as exterior/convex surfaces, as well as specific adaptations of these techniques to imaging ear canals, human dentition, and so forth.

Layered medium for three-dimensional imaging

The present invention provides, among other things, methods and systems for characterizing multifunctional objects using a flow-through device, such as, a flow cytometer. In some embodiments, an inventive method according to the present invention includes one more steps of (a) interrogating a plurality of objects (e.g., particles), wherein each individual object (e.g., particle) comprises one or more interrogation regions detectable as a sequence of events; (b) recording multiple events, wherein each individual event corresponds to each individual interrogation region detectable above a pre-determined triggering threshold; (c) grouping the recorded multiple events, and (d) characterizing the plurality of objects based on the grouped events.

Scanning multifunctional particles

Three-dimensional imaging using a single camera

Three-dimensional imaging using a fluorescent medium

Imaging tray with medium disposed therein for ratiometric determination of object shape

Three-dimensional imaging using an inflatable membrane and an intensity ratio based on differential light absorption at two wavelengths

Three-dimensional imaging using a luminescent surface and a differentially attenuating medium

Three-dimensional imaging using absorption

Growing community of inventors

Wellesley, MA, United States of America

Davide M Marini